The prevention of the occurence of ultraviolet B (UVB) induced hypoxanthine guanidine phosphoribosyl transferase (HGPRT) mutant cells by several commercial sunscreens An in vitro study

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 1998
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/20892/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=3750
• Institution: Universitas Gadjah Mada

ABSTRACT This study was aimed at comparing the effect of three different SPF (Sun Protecting Factors) of sunscreens in the prevention of UVB (Ultraviolet B) Induced mutant fibroblast cells. The study was done using a simple experimental study design. Three commercially sunscreens were coated on the plate cover surface of fibroblast culture wells. Fibroblasts were isolated from ten young boy foreskins and subcultured in 3 to 5 passages. Mutagenic transformation was done by Irradiation with 6.5 J/m2 Coerman Solarium CTL 3111 as a source of UVB. In addition there were two groups which were unprotected for control group and untreated with UVB for counting cumulative population doubling ratio (cpd). Three days after UVB irradiation, the culture, except for an unirradiated group, were incubated in HGPRT (hypoxanthine guanidine phosphoribosyl transferase) enriched medium, and fibroblast which survived in those medium considered as mutant cells. Cpd was calculated based on cell multiplication of the untreated group. The ratio of mutant cell population (mcp) is quantified by dividing the number of mutant cells with cpd. The difference of mean mcp between three subgroup of sunscreens and unprotected group was analyzed with Student's t test, and the difference of mean mcp among three subgroup of sunscreens was analyzed with ANOVA. The result showed that the mean mcp of sunscreen covered cultures were lower compared to the uncovered cultures and the higher SPF had lower mean of mcp significantly (P). Therefore sunscreen was proven to protect the induction of HGPRT mutant fibroblast although the protection was not totally, and the higher SPF sunscreen showed higher protection. Key words : sunscreens - SPF - UVB - HGPRT gene mutation - fibroblast culture. Penelitian ini bertujuan untuk membandingkan daya cegah beberapa macam tabirsurya yang memiliki SPF (Sun Protecting Factor atau Faktor Pelindung Surya) berbeda, terhadap timbulnya fibroblas mutan yang diinduksi oleh sinar ultraviolet B (UVB) secara in vitro. Penelitian dilakukan dengan desain eksperimental sederhana, yaitu 3 macam tabirsurya dengan SPF yang berbeda, dioleskan pada permukaan penutup lempeng sumuran biakan fibroblas. Fibroblas yang digunakan pada penelitian ini adalah fibroblas yang diisolasi dart 10 kulit preputium anak-anak, kemudian dilakukan subkultur 3-5 kali. Mutasi dilakukan dengan penyinaran spektrum Ultraviolet B yang berasal dari lampu Coerman solarium CTL 3111 dengan dosis 6,5 J/m2. Selain kelompok yang dilindungi oleh 3 macam tabirsurya tersebut, ditambahkan juga satu kelompok yang tidak dilindungi tabirsurya sebagai kontrol dan satu kelompok lagi yang tidak mendapat penyinaran untuk menghitung angka cpd (commutative population doubling ratio - pengganda populasi sel kumulatif). Tiga had setelah penyinaran. pada medium biakan fibroblas, kecuall yang tidak mendapat penyinaran, ditambahkan hypoxanthine guanidine phosphoribosyl transferase (HGPRT). Fibroblas yang masih tetap hidup pada media baru ini, dianggap sebagai fibroblas yang telah mengalami mutasi. Angka cpd dihitung berdasarkan jumlah sel hidup pada kelompok yang tidak mendapat penyinaran, dan angka populasi sel mutan atau mcp (mutant cell population ratio) dihitung dengan membagi jumlah sel mutan dengan cpd. Perbedaan rerata mcp antara ketiga kelompok tabir surya dengan kelompok kontrol dianalisis dengan menggunakan uji t, sedangkan perbedaan rerata mcp antara ketiga macam tabir surya dianalisis dengan menggunakan ANOVA. Hasil penelitian menunjukkan bahwa rerata mcp antara kelompok yang dilindungi oleh tabir surya dengan kelompok kontrol berbeda secara bermakna (p