PURIFICATION OF EXOINULINASE BIFIDOBACTERIUM BREVE (PEMURNTAN EKSOINULINASE DARI BIFIDOBACTERIUM BREVE)
ABSTRACT Extracellular exoinulinase was produced from Bifidobacterium breve in media with inulin as the only C-source. The enzyme was purified on DEAE-Sephadex A-50, preparative IEF and gel filtration yielded a purification of 68-fold. The pI range was from 4.2 to 4.9, the molecular weight was ident...
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| Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2000
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| Online Access: | https://repository.ugm.ac.id/21482/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=4344 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | ABSTRACT
Extracellular exoinulinase was produced from Bifidobacterium breve in media with inulin as the only C-source. The enzyme was purified on DEAE-Sephadex A-50, preparative IEF and gel filtration yielded a purification of 68-fold. The pI range was from 4.2 to 4.9, the molecular weight was identified using polyacrylamide gel electrophoresis (SDS-PAGE) and the result was 64 |
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