ANTIFUNGI PENGHAMBAT Fusarium oxysporum f.sp. cubense YANG DISINTESIS OLEH FUNGI ENDOFIT = Fusarium oxysporum f.sp. cubense INHIBITING ANTIFUNGI SYNTHESIZED BY ENDOPHYTIC FUNGI

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2002
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/22486/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=5398
• Institution: Universitas Gadjah Mada

Endophytic microbial diversity of plants in Lombok, West of Nusa Tenggara and Taman Kyai Langgeng, Magelang, Central Jawa were studied for producing antifungal compounds and controlling of pathogenic fungi, Fusarium oxysporum f.sp. cubense. The scopes of research were isolation, selection of endophytic fungi, bioassay for inhibition test, and detection of the antifungal compounds using chromatography technique. Sixty one strains of fungi were isolated from trunks and twigs of tissue plant, and were tested on their ability to produce antifungal compounds on specific media, namely the F-4, PDY (potato dextrose and yeast extract) and GY (glucose and yeast extract) media. The results showed that 19 isolates were able to inhibit F. oxysporum f.sp. cubense with diameter of inhitory zone were ranged from 12 to 21 mm. Two isolates of LEN-1 and BLW-2 had diameter of inhitory zone close to 20 mm and the best medium for producing antifungal compounds was F-4. These strains were isolated from the twigs of Euphoria longana (Kelengkengind) and Averhoa bilimbi (Belimbing wuluhInd). Preliminary characterization of antifungal compounds of LEN-1 and KBM-5 isolates using paper chromatography technique under water saturated acetic acid developer, showed the Rf : 0.39. Under the butanol : acetic acid : water (3:1 :1) developer they have Rf value 0.38. These compounds indicate good prospects for application in the agricultural field as biological control agent. Key words : Antifungi, Fusarium axysporum f.sp. cubense, Endophytic fungi.