Kloning gen coat protein smv dengan pendekatan pcr: (Cloning of the coat protein gene of smv with the pcr approach )

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 1996
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/24780/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=7758
• Institution: Universitas Gadjah Mada

ABSTRACT SMV is RNA virus had to be converted to the first strand DNA using oligo (dT) and murine reverse transcriptase. Amplification of coat protein gene region was carried out by Polimerase Chain Reaction (PCR) with two primers. The PCR product was blund ended by SI nuclease, and ligated into Sma...