Effect of Green Fluorescent Protein (GFP) on the Development of Canine Intergeneric Embryo= Pengaruh Green Fluorescent Protein (GFP) Pada Perkembangan Anjing Dari Embryo Intergeneris.
The present study investigated the effect of green fluorescent protein on the development of canine intergeneric clone embryo with bovine oocyte recipient. Cumulus oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 10% (v/v) fetal bovine serum (FBS) (...
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| Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2006
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| Online Access: | https://repository.ugm.ac.id/25711/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=8711 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | The present study investigated the effect of green fluorescent protein on the development of canine intergeneric clone embryo with bovine oocyte recipient. Cumulus oocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 10% (v/v) fetal bovine serum (FBS) (Life Technologies), 0.005 U/m1 bovine FSH (Antrin®, Denka Kanagawa, Japan) and 1 pg/m1 estradiol (Sigma-Aldrich) at 39 °C in a humidified atmosphere of 5% CO2 in air and donor cell tranfected with enhanced green fluorescent protein. In this experiment GFP have no negative effect in fusion or embryo development but the expression rate were decreased in 2 cell stage and then the expression gradually decreased with progression of embryo development.
Key words: intergeneric, clone embryo, canine
Penelitian ini meneliti pengaruh dari protein yang berpendar hijau (GFP) pada perkembangan dari kloning embrio anjing antargenus dengan menggunakan oosit sapi. Kesatuan cumulus-oosit sapi berasal dari rumah potong hewan dan dimasakkan di dalam media TCM-199 yang ditambahi dengan 10% FBS (Life technology), 0,005 Wail FSH sapi (Antrin®, Denka Kanagawa, Japan) dan 1 pg/m1 estradiol (Sigma-Aldrich) pada 39°C dalam kelembaban udara 5% CO2 dan sel donor ditempeli dengan protein yang berpendar hijau. Dalam penelitian ini, GFP tidak mempunyai pengaruh yang negatif dalam penyatuan atau perkembangan embrio, tetapi laju gambarannya menurun dalam stadium 2 sel dan secara perlahan menurun seiring perkembangan dari embrio.
Kata kunci: intergenerik, klon embrio, anjing |
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