Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase

This research is aimed to increase cellulase secretion on Trichoderma sp. by mutation method. Trichoderma PK1J2 (5x10(8) spore/ml) was turned into a suspension in utagen solution (400ug/ml ethidium bromide and 0,5 M ethyl methane sulfonate) for 120 minutes. The sreening of mutants which were able to...

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Main Authors: Mursyid, Ali, Cahyanto, M. Nur, Sardjono, Sardjono, Zuprizal, Zuprizal, Bachruddin, Zaenal
Format: Article PeerReviewed
Language:English
Published: Fak. Kedokteran Hewan Universitas Airlangga 2007
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Online Access:https://repository.ugm.ac.id/273714/1/Mutasi%20Trichoderma%20sp.%20untuk%20Meningkatkan%20Sekresi%20Selulase.pdf
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Institution: Universitas Gadjah Mada
Language: English
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spelling id-ugm-repo.2737142017-09-06T01:11:51Z https://repository.ugm.ac.id/273714/ Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase Mursyid, Ali Cahyanto, M. Nur Sardjono, Sardjono Zuprizal, Zuprizal Bachruddin, Zaenal Animal Production Animal Breeding This research is aimed to increase cellulase secretion on Trichoderma sp. by mutation method. Trichoderma PK1J2 (5x10(8) spore/ml) was turned into a suspension in utagen solution (400ug/ml ethidium bromide and 0,5 M ethyl methane sulfonate) for 120 minutes. The sreening of mutants which were able to produce hyper cellulase secretion was done by spreading it on a solid medium containing 1% carboxy methyl cellulose as the single carbon source and 15% glycerol as the catabolite repressor. Mutants which were resistant to catabolite repression and produced hyper cellulase secretion would frm larger clear zone than its parent strain after it was flooded by 1% congo red indicator. Mutant AA1, AA2, AB1, AB2, and AC1 were chosen in this screening. The cellulase activity of those five mutants was measured after it was inoculated in liquid medium for 4 days. Cellulase activity measurement was done by using number 1 Whatmann filter paper substrate. From the five mutants, mutant AA1 gave highest cellulase activity. The mutant AA1 and its parent strain were inoculated in liquid medium for 7 days and its cellulase activity was measured every day. The peak of cellulase activity was reached after being incubated for 5 and 7 days for mutant AA1 and its parent strain respectively. The maximum cellulase activity of mutant AA1 was 1.4 times higher than its parent strain. It is concluded that mutation on Trichoderma sp. produces mutant which is resistant to catabolite repression ant is able to produce hyper cellulase secretion. Fak. Kedokteran Hewan Universitas Airlangga 2007-05 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/273714/1/Mutasi%20Trichoderma%20sp.%20untuk%20Meningkatkan%20Sekresi%20Selulase.pdf Mursyid, Ali and Cahyanto, M. Nur and Sardjono, Sardjono and Zuprizal, Zuprizal and Bachruddin, Zaenal (2007) Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase. Media Kedokteran Hewan, 23 (2). pp. 74-79. ISSN 2015-8930
institution Universitas Gadjah Mada
building UGM Library
country Indonesia
collection Repository Civitas UGM
language English
topic Animal Production
Animal Breeding
spellingShingle Animal Production
Animal Breeding
Mursyid, Ali
Cahyanto, M. Nur
Sardjono, Sardjono
Zuprizal, Zuprizal
Bachruddin, Zaenal
Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
description This research is aimed to increase cellulase secretion on Trichoderma sp. by mutation method. Trichoderma PK1J2 (5x10(8) spore/ml) was turned into a suspension in utagen solution (400ug/ml ethidium bromide and 0,5 M ethyl methane sulfonate) for 120 minutes. The sreening of mutants which were able to produce hyper cellulase secretion was done by spreading it on a solid medium containing 1% carboxy methyl cellulose as the single carbon source and 15% glycerol as the catabolite repressor. Mutants which were resistant to catabolite repression and produced hyper cellulase secretion would frm larger clear zone than its parent strain after it was flooded by 1% congo red indicator. Mutant AA1, AA2, AB1, AB2, and AC1 were chosen in this screening. The cellulase activity of those five mutants was measured after it was inoculated in liquid medium for 4 days. Cellulase activity measurement was done by using number 1 Whatmann filter paper substrate. From the five mutants, mutant AA1 gave highest cellulase activity. The mutant AA1 and its parent strain were inoculated in liquid medium for 7 days and its cellulase activity was measured every day. The peak of cellulase activity was reached after being incubated for 5 and 7 days for mutant AA1 and its parent strain respectively. The maximum cellulase activity of mutant AA1 was 1.4 times higher than its parent strain. It is concluded that mutation on Trichoderma sp. produces mutant which is resistant to catabolite repression ant is able to produce hyper cellulase secretion.
format Article
PeerReviewed
author Mursyid, Ali
Cahyanto, M. Nur
Sardjono, Sardjono
Zuprizal, Zuprizal
Bachruddin, Zaenal
author_facet Mursyid, Ali
Cahyanto, M. Nur
Sardjono, Sardjono
Zuprizal, Zuprizal
Bachruddin, Zaenal
author_sort Mursyid, Ali
title Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
title_short Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
title_full Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
title_fullStr Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
title_full_unstemmed Mutasi Trichoderma sp. untuk Meningkatkan Sekresi Selulase
title_sort mutasi trichoderma sp. untuk meningkatkan sekresi selulase
publisher Fak. Kedokteran Hewan Universitas Airlangga
publishDate 2007
url https://repository.ugm.ac.id/273714/1/Mutasi%20Trichoderma%20sp.%20untuk%20Meningkatkan%20Sekresi%20Selulase.pdf
https://repository.ugm.ac.id/273714/
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