Direct Stimulation by Methanol Addition on the Cultured Medium for Methanol Dehydrogenase Protein Purification from Bradyrhizobium japonicum USDA110

• Main Authors: Kurniawati, Novita, Pertiwiningrum, Ambar, Erwanto, Yuny, Fitriyanto, Nanung Agus, Abidin, Muhammad Zainal
• Format: Article PeerReviewed
• Language: English; English
• Published: Faculty of Animal Science, Universitas Gadjah Mada 2018
• Subjects: Biochemistry and Cell Biology; Enzymes; Microbiology; Biological Sciences
• Online Access: https://repository.ugm.ac.id/274932/1/28155-100065-1-PB.pdf; https://repository.ugm.ac.id/274932/2/21973; https://repository.ugm.ac.id/274932/; http://buletinpeternakan.fapet.ugm.ac.id/
• Institution: Universitas Gadjah Mada

Methanol dehydrogenase (MDH) enzyme was purified from Bradyrhizobium japonicum USDA110 cell-free extract. The bacteria were grown in a culture medium with direct 0.5% methanol addition aimed to stimulates the MDH catalytic enzyme activation. Bradyrhizobium japonicum USDA110 MDH enzyme was purified b...