Pemurnian dan Deteksi Serologi Patchouli Mottle Virus pada Tanaman Nilam
Patchouli mottle virus (PatMo V) is the most severe disease pathogen and causes substantial losses in many patchouli-producing regions in Indonesia. Serological detection tool for the disease was developed in this research. Virus isolation was conducted on Chenopodium amaraticolor resulted on the ho...
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| Format: | Article NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2006
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| Online Access: | https://repository.ugm.ac.id/27538/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10598 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Patchouli mottle virus (PatMo V) is the most severe disease pathogen and causes substantial losses in many patchouli-producing regions in Indonesia. Serological detection tool for the disease was developed in this research. Virus isolation was conducted on Chenopodium amaraticolor resulted on the homogenous local lesions 6 days after inoculation. Virus purification was obtainedfrom 200g inoculated leaves resulted on 2 ml virus solution with the concentration of 1 mg/ml. Polyclonal antibodies were produced on rabbits.
Harvested antiserum was used for further virus detection by Indirect-Enzyme linked Immunosorbent assay (I-ELISA) and dot-immunobanding assay (DIBA) techniques. The
antibodies were positively reacted with purified viruses, infected field collection of patchouli, and inoculated C. amaranticolor. On the other hand un-inoculated C. amaranticolor samples and healthy patchouli generated from tissue cultures gave negative reaction with the
antibodies. This is the first report of cheap practical antibody production for PatMo V detection in Indonesia.
Key words: Patchouli, polyclonal antibody, Patchouli mottle virus, ELISA, DIBA. |
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