Detection of polymorphism in growth differentiation factor 9 (GDF9) gene in Indonesian goats
A member of growth factor and a member of the transforming growth factor β superfamily, growth differentiation factor 9 (GDF9) has a critical role in prolificacy of sheep and goat. The aim of this study was to analyze the exon 1 of GDF9 gene in Indonesian goats. For this purpose, 78 blood samples...
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| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item PeerReviewed |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/276051/1/ICOFA%20DM%20-%20goat%20GDF9.pdf https://repository.ugm.ac.id/276051/ |
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| Institution: | Universitas Gadjah Mada |
| Language: | English |
| Summary: | A member of growth factor and a member of the transforming growth
factor β superfamily, growth differentiation factor 9 (GDF9) has a critical role in
prolificacy of sheep and goat. The aim of this study was to analyze the exon 1 of
GDF9 gene in Indonesian goats. For this purpose, 78 blood samples representing ten
Indonesian goat breeds: Gembrong (GB), Senduro (SD), Peranakan Etawah (PE),
Boerawa (BR), Boerka (BK), Kosta (KT), Muara (MR), Boer (BO), Samosir (SM)
and Kacang (KC) were collected. An exonic region of GDF9 gene, 462 bp was
amplified using specific primers. Two SNPs were identified based on reference and
direct sequencing, SNP 306G>A and SNP 239C>A, respectively. SNP 306G>A was
used to genotype all animals using RFLP method with HhaI restriction enzyme. The
results revealed an absence of polymorphism of GDF9 gene at SNP 306G>A in all
animals investigated. Hence, a further attempt should be addressed to investigate this
SNP 239C>A of GDF9 gene which may influence the prolificacy of goats. |
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