EFFECTOF HIRA PROTEINONTRANSCRIPTIONALREGULATIONSOF GENESIN VERTEBRATECELLS
ABSTRACT For better understanding of DNA replicating-coupled chromatin assembly and transcription regulation in eukaryotes, we studied biochemical and genetic analysis of nuclear-related proteins from chicken DT40 cell lines. The genetic analysis of some nuclear proteins, such as HIRA and CAF-1, ind...
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| Format: | Article NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2008
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| Online Access: | https://repository.ugm.ac.id/27944/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11007 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | ABSTRACT
For better understanding of DNA replicating-coupled chromatin assembly and transcription regulation in
eukaryotes, we studied biochemical and genetic analysis of nuclear-related proteins from chicken DT40 cell lines.
The genetic analysis of some nuclear proteins, such as HIRA and CAF-1, indicated that these proteins could play
overlappingroles in chromatin dynamics and is consistent with the finding that HIRA protein exhibited binding ability
to histones and ASF-1, as also ASF-1 bound directly with CAF-1p60. In this study, revealed not only that the Nterminal
and C-terminal halves of HIRA mediate individually transcription repressions but also that even one of the
sevenWDdipeptide motifs and the LXXLL motif of HIRA are required for these mediations in vivo. Finally,we found
that HIRA-mediatedrepression is sensitive to tricostatin TSAand it co-represses transcription together with HDAC-2.
We believe our findings will contribute to a major break-throughin future studies on the specific, individual roles of
HIRA involved in numerous DNA-utilizing processes, through the formation and/or maintenance of the chromatin
structurein vertebratecells.
Keywords: histone regulator, transcriptionalrepression,tricostatin,chromatin |
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