BIOSURFACTANTS PRODUCTION BY Pseudomonas aeruginosa USING SOYBEAN OIL AS SUBSTRATE
ABSTRACT Optimization condition of the biosurfactants production by P. aeruginosa using soybean oil as substrate has been examined. The media containing 10% v/v of the soybean oil and 6 days of the fermentation time was the optimum condition for the biosurfactants production. The extraction techniqu...
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| Format: | Article NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2008
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/27978/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11041 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | ABSTRACT
Optimization condition of the biosurfactants production by P. aeruginosa using soybean oil as substrate has
been examined. The media containing 10% v/v of the soybean oil and 6 days of the fermentation time was the
optimum condition for the biosurfactants production. The extraction technique using different solvent polarity (nhexane,
chloroform, ethyl acetate and buthanol, respectively) was applied for the isolation of the biosurfactants. The
biosurfactant was found in the extract chloroform of the crude biospasoy (biosurfactants obtained from soybean oil
as substrate) which then is called chlo-biospasoy. The chlo-biospasoy was identified as rhamnolipids which had oil
in water (oIw) emulsion type, had the CMC of 860 mglL and could reduced the surface tension of the water from 72
mN/m to 52 mN/m. The chlo-biospasoy could be used as an emulsifier to form emulsion between water and
hydrocarbon such as palm oil, benzene, premium or toluene with various stability. The results indicated that chlobiospasoy
could be used as an emulsifying and emulsion-stabilizing agent. |
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