HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC DETERMINATION OF CAFFEIC ACID AND ROSMARINIC ACID FROM THE LEAVES OF Orthosiphon stamineus
ABSTRACT This paper presents the studies performed on extraction of Orthosiphon stamineus, Benth by using different solvent for the identification and quantification of the caffeic acid derivatives such as caffeic acid and rosmarinic acid which confers to the leaves of this plant with remarkable ph...
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| Format: | Article NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2008
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| Online Access: | https://repository.ugm.ac.id/27983/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11046 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | ABSTRACT
This paper presents the studies performed on extraction of Orthosiphon stamineus, Benth by using different
solvent for the identification and quantification of the caffeic acid derivatives such as caffeic acid and rosmarinic acid
which confers to the leaves of this plant with remarkable pharmaceutical properties. High performance thin-layer
chromatographic (HPTLC) allows the identification and the quantification of more than 20 samples in the same
chromatographic run. The analysis of the samples requires 15-30 min compared with more than 2 h using a typical
HPLC method. Using the techniques of the HPTLC and the UV-VIS spectra we have found that the extraction of this
herb plant contain, the caffeic acid and rosmarinic acid ranging between 0.029% up to 0.506% and up to 0.24% to
2.24% respectively. |
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