UTILIZATIONOF MEMBRANE MICROFILTRATIONIN PREPARATION OF HYDROLYZED VEGETABLEPROTEINFROMFERMENTEDREDBEAN(Phaseolus vulgaris L.) EXTRACT AS FORTIFICATIONAGENT

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2009
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/28017/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11080
• Institution: Universitas Gadjah Mada

ABSTRACT Preparation of Hydrolyzed Vegetable Protein (HVP) as savory flavor from fermented red bean broth through stirredmembrane cell using micro filtration membrane with pora size of 0.45 fJmwasperformed to get fortified agent utilized in preparation of beans sauce. The objective of this work was to study an effect of pressure and kind of red bean broth extract on content of total protein, soluble protein and dry solid in the retentate and permeate as hydrolyzed vegetable protein used for fortified agent of red bean sauces. Preparation process of hydrolyzed vegetableprotein was done using fixed rotary speed of 400 rpm, pressure of 20, 25 and 30 psi at room temperature. To investigate the effect of pressure on this separation, the feed were red bean broth extract fermented for 6, 8, 10 and 12 weeks, respectively. Fermentation process were conducted using salt fermentation with inoculum of Rhizopus-C1,salt and red bean ratios of 30:10:60%. The analysis of flux and contents of total protein, dissolved protein and dry solid in the retentate and permeate was carried out, and the result of experiment showed that interaction of Red bean broth extract with 6, 8, 10 and 12 weeks of fermentation and operation condition of microfiltration membrane separation tends to affect on flux and content of total protein, dissolved proteIn and dry solid in retentate and permeate. Red bean broth extract for 6 weeks fermentation resulted higher protein content in permeate as o/..drolyzedvegetable protein than in retentate. Permeate at pressure of 25 psi gives flux value of 0.0217 mUcm .minute and contents of total protein of 1.31 %, dissolved protein of 6.9 mg/g, and dry solid of 2.6%, while retentate as hydrolyzed vegetable protein or fortified agent indicate contents of total protein of 1.52%, dissolved protein of 4.15mg/g, and dry solid of 3.64%. It was found that micro filtrationprocess was able to increase dissolvedprotein content of about 3 times. Keywords: Microfiltration, membrane, Hydrolyzed Vegetable Protein (HVP), fermented red bean (phaseolus vulgaris L.)