FRAGMENTATION STUDIES OF &Delta

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2009
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/28044/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11107
• Institution: Universitas Gadjah Mada

ABSTRACT Semisynthesisof A6.7-Anhydroerythromycin-Awas done by biomodification technique by addition of 0.2% INH into a culture fermentation of Saccharopolyspora erythraea ATCC 11635 in medium Hutchinson. The aim of this research is to studies of fragmentation pattern from new matabolite of A6.7-Anhydroerythromycin-Aby Liquid Chromatography-MassSpectroscopy(LC-MS) and the ionization of mass spectroscopy is use by ESI (Electrospray Ionization) pattern. The FT-IR spectrometric analyzes showed a stretching vibration of C=C conjugated group at wave number 1602.7 cm-1.This C=C conjugated vibrationindicated the existence of double bond between C6and C7 (A6.7),this confirmedthat isolate containedA6.7-Anhydroerythromycin(t-hAe possibilityof A6.7was positive).For complementation, a LC-MS (Liquid Chromatography-Mass Spectroscopy) analyzes using ESI-MS (Electrospray Ionization-Mass Spectroscopy) ionization pattern was conducted to the isolate which resulted Quassimolecularions [M+Hf of A7.8_and A6.7-AnhydroerythromycinL-CA-.MSspectrogramof the isolate,whichgave twopeaksof m/z 732.2460 and m/z 716.2522, confirmed that the m/z 732.2460possibly was A7.8-Anhydroerythromycin-A, while the m/z 716.2502andm/z 715.2522possibly were A6.7-Anhydroerythromycin-A. Keywords: isoniazid, enoyl reduction, A6,.7-Anhidroeritromisin-Afr, agmentation,LC-MS.