ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM
ABSTRACT Some factors such as the changes of the stationary phase, temperature,pH-value, mobile-phase composition and flow rate play a crucial role in effecting the sensitivity of retention times in high performance liquid chromatography (HPLC) system. Utilizing a retention index system is one of th...
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2009
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id-ugm-repo.280572014-06-18T00:24:18Z https://repository.ugm.ac.id/28057/ ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Some factors such as the changes of the stationary phase, temperature,pH-value, mobile-phase composition and flow rate play a crucial role in effecting the sensitivity of retention times in high performance liquid chromatography (HPLC) system. Utilizing a retention index system is one of the methods to minimize those effects. Besides the mentioned factors, dead-time influences on determining the retention index as well. In comparison with Gas Chromatography (GC), the retention Index determination method in HPLC is still widely discussed, due to the difficulty of utilizing n-alkane as standard. In addition, the solutes in HPLC interact with the mobile-phase, thus the retention behavior also depend on the mobile-phase.Actually,"It is difficult to use n-alkanes in HPLC as standards in case of some considerable problems, due to they are very non polar but also large retention times which lack of chromophores. Therefore, using n-alkane in routine analysis could be inconvenient. In comparison with n-alkanes, the alkylarylketones homologous series are stable compounds, commercially available and easily detected by a UV detector. This paper introduces Determination of Kovats Retention Index in the HPLC using Alkylarylketone homologous series and then is connected with n-alkane as a frame of reference. Steroids were used as test substance for calculating Kovats retention index values in cetonitrile/watersystem. Keywords: Kovats Retention Index, RP- HPLC, n-alkane, alkylarylketone [Yogyakarta] : Universitas Gadjah Mada 2009 Article NonPeerReviewed Perpustakaan UGM, i-lib (2009) ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11120 |
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ABSTRACT
Some factors such as the changes of the stationary phase, temperature,pH-value, mobile-phase composition
and flow rate play a crucial role in effecting the sensitivity of retention times in high performance liquid
chromatography (HPLC) system. Utilizing a retention index system is one of the methods to minimize those effects. Besides the mentioned factors, dead-time influences on determining the retention index as well. In comparison with Gas Chromatography (GC), the retention Index determination method in HPLC is still widely discussed, due to the difficulty of utilizing n-alkane as standard. In addition, the solutes in HPLC interact with the mobile-phase, thus the retention behavior also depend on the mobile-phase.Actually,"It is difficult to use n-alkanes in HPLC as standards in
case of some considerable problems, due to they are very non polar but also large retention times which lack of chromophores. Therefore, using n-alkane in routine analysis could be inconvenient. In comparison with n-alkanes, the alkylarylketones homologous series are stable compounds, commercially available and easily detected by a UV detector. This paper introduces Determination of Kovats Retention Index in the HPLC using Alkylarylketone homologous series and then is connected with n-alkane as a frame of reference. Steroids were used as test substance for calculating Kovats retention index values in cetonitrile/watersystem.
Keywords: Kovats Retention Index, RP- HPLC, n-alkane, alkylarylketone |
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Article NonPeerReviewed |
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Perpustakaan UGM, i-lib |
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Perpustakaan UGM, i-lib |
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Perpustakaan UGM, i-lib |
title |
ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM |
title_short |
ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM |
title_full |
ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM |
title_fullStr |
ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM |
title_full_unstemmed |
ALKYLARYLKETONEHOMOLOGOUS SERIES FOR DETERMINATIONOF KOVATS RETENTION INDICES WITH RP-HPLC USING ACETONITRILEIWATER SYSTEM |
title_sort |
alkylarylketonehomologous series for determinationof kovats retention indices with rp-hplc using acetonitrileiwater system |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2009 |
url |
https://repository.ugm.ac.id/28057/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11120 |
_version_ |
1681219076131651584 |