Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia
Introduction. A global surge in SARS-CoV-2 cases is occurring due to the emergence of new disease variants, and requires continuous adjustment of public health measures. This study aims to continuously monitor and mitigate the impact of SARS-CoV-2 through genomic surveillance, to determine the emerg...
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id-ugm-repo.2830372023-11-17T06:12:31Z https://repository.ugm.ac.id/283037/ Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia Massi, Muhammad Nasrum Abidin, Rufika Shari Farouk, Abd-ElAziem Halik, Handayani Soraya, Gita Vita Hidayah, Najdah Sjahril, Rizalinda Handayani, Irda Hakim, Mohamad Saifudin Gazali, Faris Muhammad Setiawaty, Vivi Wibawa, Tri Clinical Microbiology Medical Microbiology Introduction. A global surge in SARS-CoV-2 cases is occurring due to the emergence of new disease variants, and requires continuous adjustment of public health measures. This study aims to continuously monitor and mitigate the impact of SARS-CoV-2 through genomic surveillance, to determine the emergence of variants and their impact on public health. Methods. Data were collected from 50 full-genome sequences of SARS-CoV-2 isolates from Makassar, South Sulawesi, Indonesia. Mutation and phylogenetic analysis was performed of SARS-CoV-2 from Makassar, South Sulawesi, Indonesia. Results. Phylogenetic analysis showed that two samples (4%) were of the B.1.319 lineage, while the others (96%) were of the B.1.466.2 lineage. Mutation analysis of the spike (S) protein region showed that the most common mutation was D614G (found in 100% of the sequenced isolates), followed by N439K (98%) and P681R (76%). Several mutations were also identified in other genomes with a high frequency, including P323L (nsp12), Q57H (ns3-orf3a), and T205I (nucleoprotein). Conclusion. Our findings highlight the importance of continuous genomic surveillance to identify new viral mutations and variants with possible impacts on public health. PeerJ Inc. 2022-06-10 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283037/1/Hakim_KKMK.pdf Massi, Muhammad Nasrum and Abidin, Rufika Shari and Farouk, Abd-ElAziem and Halik, Handayani and Soraya, Gita Vita and Hidayah, Najdah and Sjahril, Rizalinda and Handayani, Irda and Hakim, Mohamad Saifudin and Gazali, Faris Muhammad and Setiawaty, Vivi and Wibawa, Tri (2022) Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia. PeerJ, 10 (e1352). pp. 1-18. ISSN 21678359 https://peerj.com/articles/13522/ https://doi.org/10.7717/peerj.13522 |
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Clinical Microbiology Medical Microbiology Massi, Muhammad Nasrum Abidin, Rufika Shari Farouk, Abd-ElAziem Halik, Handayani Soraya, Gita Vita Hidayah, Najdah Sjahril, Rizalinda Handayani, Irda Hakim, Mohamad Saifudin Gazali, Faris Muhammad Setiawaty, Vivi Wibawa, Tri Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
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Introduction. A global surge in SARS-CoV-2 cases is occurring due to the emergence of new disease variants, and requires continuous adjustment of public health measures. This study aims to continuously monitor and mitigate the impact of SARS-CoV-2 through genomic surveillance, to determine the emergence of variants and their impact on public health. Methods. Data were collected from 50 full-genome sequences of SARS-CoV-2 isolates from Makassar, South Sulawesi, Indonesia. Mutation and phylogenetic analysis was performed of SARS-CoV-2 from Makassar, South Sulawesi, Indonesia. Results. Phylogenetic analysis showed that two samples (4%) were of the B.1.319 lineage, while the others (96%) were of the B.1.466.2 lineage. Mutation analysis of the spike (S) protein region showed that the most common mutation was D614G (found in 100% of the sequenced isolates), followed by N439K (98%) and P681R (76%). Several mutations were also identified in other genomes with a high frequency, including P323L (nsp12), Q57H (ns3-orf3a), and T205I (nucleoprotein). Conclusion. Our findings highlight the importance of continuous genomic surveillance to identify new viral mutations and variants with possible impacts on public health. |
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Article PeerReviewed |
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Massi, Muhammad Nasrum Abidin, Rufika Shari Farouk, Abd-ElAziem Halik, Handayani Soraya, Gita Vita Hidayah, Najdah Sjahril, Rizalinda Handayani, Irda Hakim, Mohamad Saifudin Gazali, Faris Muhammad Setiawaty, Vivi Wibawa, Tri |
author_facet |
Massi, Muhammad Nasrum Abidin, Rufika Shari Farouk, Abd-ElAziem Halik, Handayani Soraya, Gita Vita Hidayah, Najdah Sjahril, Rizalinda Handayani, Irda Hakim, Mohamad Saifudin Gazali, Faris Muhammad Setiawaty, Vivi Wibawa, Tri |
author_sort |
Massi, Muhammad Nasrum |
title |
Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
title_short |
Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
title_full |
Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
title_fullStr |
Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
title_full_unstemmed |
Full-genome sequencing and mutation analysis of SARS-CoV-2 isolated from Makassar, South Sulawesi, Indonesia |
title_sort |
full-genome sequencing and mutation analysis of sars-cov-2 isolated from makassar, south sulawesi, indonesia |
publisher |
PeerJ Inc. |
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2022 |
url |
https://repository.ugm.ac.id/283037/1/Hakim_KKMK.pdf https://repository.ugm.ac.id/283037/ https://peerj.com/articles/13522/ https://doi.org/10.7717/peerj.13522 |
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1783956361476833280 |