Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma

Basal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cel...

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Main Authors: Ferronika, Paranita, Dhiyani, Safira Alya, Budiarti, Tri, Widodo, Irianiwati, Rinonce, Hanggoro Tri, Anwar, Sumadi Lukman
Format: Article PeerReviewed
Language:English
Published: MDPI 2022
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Online Access:https://repository.ugm.ac.id/283173/1/20.pdf
https://repository.ugm.ac.id/283173/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144832564&doi=10.3390%2fdiagnostics12122987&partnerID=40&md5=2ed77a43fc0b28eba80dd447df664627
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spelling id-ugm-repo.2831732023-11-20T02:02:01Z https://repository.ugm.ac.id/283173/ Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma Ferronika, Paranita Dhiyani, Safira Alya Budiarti, Tri Widodo, Irianiwati Rinonce, Hanggoro Tri Anwar, Sumadi Lukman Cancer Diagnosis Basal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cells (Treg cells) in the tumour centre (TC), tumour periphery (TP), and normal adjacent tissue (NAT) of BCC. A total of 111 samples from 43 cutaneous BCC cases were examined for TIL (CD3+) and Treg cell (FOXP3+/CD3+) expression using immunohistochemical techniques. The correlations of Treg cells with TILs, invasion depth, and tumour morphological risk were analysed. We identified a high mean proportion of Treg cells within the tumour (TC = 46.9, TP = 56.1, NAT = 51.8) despite a relatively low median of TILs (TC = 12.7, TP = 10.3, NAT = 3.6), supporting the classification of BCC as a cold tumour. A significant positive correlation was observed between the proportion of Treg cells and sTILs (ρ = 0.325, p < 0.001), suggesting a predominant role of TILs in the infiltration of Treg cells. An inverse correlation discovered between Treg cells and tumour invasion depth (r = −0.36, p = 0.017) might indicate Treg cells’ anti-tumour capacity in BCC. © 2022 by the authors. MDPI 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283173/1/20.pdf Ferronika, Paranita and Dhiyani, Safira Alya and Budiarti, Tri and Widodo, Irianiwati and Rinonce, Hanggoro Tri and Anwar, Sumadi Lukman (2022) Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma. Diagnostics, 12 (12). ISSN 2075-4418 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144832564&doi=10.3390%2fdiagnostics12122987&partnerID=40&md5=2ed77a43fc0b28eba80dd447df664627 10.3390/diagnostics12122987
institution Universitas Gadjah Mada
building UGM Library
continent Asia
country Indonesia
Indonesia
content_provider UGM Library
collection Repository Civitas UGM
language English
topic Cancer Diagnosis
spellingShingle Cancer Diagnosis
Ferronika, Paranita
Dhiyani, Safira Alya
Budiarti, Tri
Widodo, Irianiwati
Rinonce, Hanggoro Tri
Anwar, Sumadi Lukman
Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
description Basal cell carcinoma (BCC) is the most common skin malignancy worldwide. Current evidence suggests tumour-infiltrating lymphocytes (TILs) may influence the clinical outcomes of patients with BCC. The present study aimed to profile the infiltrative characteristics of stromal TILs and regulatory T cells (Treg cells) in the tumour centre (TC), tumour periphery (TP), and normal adjacent tissue (NAT) of BCC. A total of 111 samples from 43 cutaneous BCC cases were examined for TIL (CD3+) and Treg cell (FOXP3+/CD3+) expression using immunohistochemical techniques. The correlations of Treg cells with TILs, invasion depth, and tumour morphological risk were analysed. We identified a high mean proportion of Treg cells within the tumour (TC = 46.9, TP = 56.1, NAT = 51.8) despite a relatively low median of TILs (TC = 12.7, TP = 10.3, NAT = 3.6), supporting the classification of BCC as a cold tumour. A significant positive correlation was observed between the proportion of Treg cells and sTILs (ρ = 0.325, p < 0.001), suggesting a predominant role of TILs in the infiltration of Treg cells. An inverse correlation discovered between Treg cells and tumour invasion depth (r = −0.36, p = 0.017) might indicate Treg cells’ anti-tumour capacity in BCC. © 2022 by the authors.
format Article
PeerReviewed
author Ferronika, Paranita
Dhiyani, Safira Alya
Budiarti, Tri
Widodo, Irianiwati
Rinonce, Hanggoro Tri
Anwar, Sumadi Lukman
author_facet Ferronika, Paranita
Dhiyani, Safira Alya
Budiarti, Tri
Widodo, Irianiwati
Rinonce, Hanggoro Tri
Anwar, Sumadi Lukman
author_sort Ferronika, Paranita
title Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
title_short Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
title_full Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
title_fullStr Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
title_full_unstemmed Regulatory T Cells but Not Tumour-Infiltrating Lymphocytes Correlate with Tumour Invasion Depth in Basal Cell Carcinoma
title_sort regulatory t cells but not tumour-infiltrating lymphocytes correlate with tumour invasion depth in basal cell carcinoma
publisher MDPI
publishDate 2022
url https://repository.ugm.ac.id/283173/1/20.pdf
https://repository.ugm.ac.id/283173/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144832564&doi=10.3390%2fdiagnostics12122987&partnerID=40&md5=2ed77a43fc0b28eba80dd447df664627
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