Efficacy of C-10 massoialactone against-multispecies microbial biofilm

• Main Authors: Hamzah, Hasyrul, Pratiwi, Sylvia Utami Tunjung, Hertiani, Triana
• Format: Article PeerReviewed
• Language: English
• Published: AMG Transcend Association 2021
• Subjects: Pharmacology and Pharmaceutical Sciences
• Online Access: https://repository.ugm.ac.id/283806/1/Pratiwi_FA.pdf; https://repository.ugm.ac.id/283806/; https://www.scopus.com/record/display.uri?eid=2-s2.0-85112371633&doi=10.33263%2fBRIAC123.34723487&origin=inward&txGid=c10107b2bfecae3045238c08e0a57b18; https://doi.org/10.33263/BRIAC123.34723487
• Institution: Universitas Gadjah Mada

Biofilm is a community of Microbes found in almost all habitats. They bind to the surface and become incorporated in the biomolecule-rich extracellular matrix. The matrix can fully Microorganisms are covered and rendered more immune to antibacterial agents, becoming a progressive infection source. Anti-biofilm compounds need to be discovered to deal with these biofilm-mediated infections. C-10 Massoialactone(C10H16O2), a significant component of MassoiaaromaticaBecc. The essential oil has demonstrated that antibacterial and anti-fungal properties are possible. This study aims to see how effective C-10 Massoialactone is against multi-species microbial biofilm of Candida albicans, Pseudomonas aeruginosa, and Escherichia coli. Massoia lactone It is derived from the essential oil of Massoiaaromatica bark. To isolate and explain these compounds, researchers used preparative thin layer chromatography (TLC), gas chromatography-mass spectrometry (GC-MS), with 1D-1H nuclear magnetic resonance (NMR) analysis. The impact of horse-tail lactone on several microbial biofilms was investigated. The biofilm structure was analyzed using a transmission electron microscope (TEM) and scanning electron microscope (SEM) the biofilm structure. GCMS and NMR results revealed the presence of C-10 Massoialactone (96.59 %). C-10 Massoialactoneshowed a dosedependent activity in inhibiting biofilm formation as well as in breaking down established biofilms. Higher concentrations of C-10 Massoialactoneare required to inhibit the mature phase of the tested biofilm. TEM and SEM analysis showed apparent cell lysis in the biofilm in the presence of C-10 Massoialactone. C-10 Massoialactone can function because of its rich potential as a new anti-biofilm drug for various microbial biofilms.