Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanica on Hela cell = Spektroskopik dan aktivitas sitotoksik kuasinoid hasil isolasi dari biji buah Brucea javanica ...

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/28919/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11982
• Institution: Universitas Gadjah Mada

The quassinoids from Brucea javanica exhibit biological activities as antimalarial, antitumor, antiviral and antiamoebic activities. These quassinoids having a promising antitumor activity.This research was aimed to isolated quassinoid (bruceine A, MW 522 g/mol) compound from B. javanica and to investigate the cytotoxicity of brucetne A under invitro conditions in Hela cells. The cytotoxicity assays using MTT assay. The bruceine showed a good cytotoxic activity in Hela cells at 24 hours with doxorubicine as a positive control. Structural elucidation of bruceine is done by using UV and Ff-IR spectra, NMR spectra recorded on Tesla av600, (400 MHz) lHNMR in pyridine (Os), some in Gyro (300 MHz) lHNMR in acetone-(d6), OMSO-(d6) and CdCI3 ,13CNMR,75 MHz, mass spectra on biospectrometry LC-MS. Analytical HPLC retention time,tR was 4.617 minutes (Met/H20) 60:40. Its Molecular formula was determined to be ( C26H34011) by the [M+H]+ ion peak at m/z 523 and m/z 1045 confirmed by High Resolution mass spectra Orbitrap (HRESIMS). From the results demonstrated that could be bruceine A compound refers to our spectral data. Its has activity in ICso value 191,5 ~M on Hela Cells under invitro conditions.