The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue's Sprague dawley Rats

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/29086/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12149
• Institution: Universitas Gadjah Mada

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLCis between 400-500 nm and considered non-ionizing radiation that can produce free radicals.According to previous research,the light at wavelength < 500 nm could inhibit cells mitosis, causecells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLCexposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats.The subjects of this study consisted of ,20Spraguedawley rats, in 2-3 months of age and divided into 4 groups. Eachgroup was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st(group B), 3rd(group C), 5th(group D) day of VLCexposure, respectively. The exposure of VLCwas done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was asthick as 2 layersof celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Eachspecimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by KruskalWallis. The number of neutrophil and macrophage were analyzed by using one way ANOVA.The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Spraguedawley rats. The result of this study indicated that VLCexposure might decrease the 'thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.