EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS
Stability of erythropoietin (EPO) depends on its glycosylation states. With more glycosylation sites, the EPO protein will be more stable and also increase its half-life. A construct of recombinant human erythropoietin (rhEPO) which contains 2 additional N-link for glycosylation were designed. Based...
Saved in:
Main Author: | |
---|---|
Format: | Article NonPeerReviewed |
Published: |
[Yogyakarta] : Universitas Gadjah Mada
2012
|
Subjects: | |
Online Access: | https://repository.ugm.ac.id/29178/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12241 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Gadjah Mada |
id |
id-ugm-repo.29178 |
---|---|
record_format |
dspace |
spelling |
id-ugm-repo.291782014-06-18T00:23:05Z https://repository.ugm.ac.id/29178/ EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS Perpustakaan UGM, i-lib Jurnal i-lib UGM Stability of erythropoietin (EPO) depends on its glycosylation states. With more glycosylation sites, the EPO protein will be more stable and also increase its half-life. A construct of recombinant human erythropoietin (rhEPO) which contains 2 additional N-link for glycosylation were designed. Based on translation analysis using ORF (open reading frame)-finder and protein alignment analysis using blast-p of NCBI home page, expected recombinant hEPO with additional 6-histidin tag in carboxyl terminus was expressed. HEK293T cells were transfected with recombinant plasmid containing rhEPO by using calcium phosphate method. Expression of rhEPO was detected by dot blot and Western blot analysis using hEPO antibody as the primary antibody and antirabbit antibody with alkaline phospatase linked as the secondary antibody. The bands were detected by BCIP/NBT color development substrate. The data indicated detection of EPO in culture medium of transfected HEK293T cells. [Yogyakarta] : Universitas Gadjah Mada 2012 Article NonPeerReviewed Perpustakaan UGM, i-lib (2012) EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12241 |
institution |
Universitas Gadjah Mada |
building |
UGM Library |
country |
Indonesia |
collection |
Repository Civitas UGM |
topic |
Jurnal i-lib UGM |
spellingShingle |
Jurnal i-lib UGM Perpustakaan UGM, i-lib EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
description |
Stability of erythropoietin (EPO) depends on its
glycosylation states. With more glycosylation sites, the EPO
protein will be more stable and also increase its half-life. A
construct of recombinant human erythropoietin (rhEPO) which
contains 2 additional N-link for glycosylation were designed. Based
on translation analysis using ORF (open reading frame)-finder and
protein alignment analysis using blast-p of NCBI home page,
expected recombinant hEPO with additional 6-histidin tag in
carboxyl terminus was expressed. HEK293T cells were transfected
with recombinant plasmid containing rhEPO by using calcium
phosphate method. Expression of rhEPO was detected by dot blot
and Western blot analysis using hEPO antibody as the primary
antibody and antirabbit antibody with alkaline phospatase linked
as the secondary antibody. The bands were detected by
BCIP/NBT color development substrate. The data indicated
detection of EPO in culture medium of transfected HEK293T cells. |
format |
Article NonPeerReviewed |
author |
Perpustakaan UGM, i-lib |
author_facet |
Perpustakaan UGM, i-lib |
author_sort |
Perpustakaan UGM, i-lib |
title |
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
title_short |
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
title_full |
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
title_fullStr |
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
title_full_unstemmed |
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS |
title_sort |
expression of recombinant human erythropoietin with glycosylation modification in hek293t cells |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2012 |
url |
https://repository.ugm.ac.id/29178/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12241 |
_version_ |
1681219287079976960 |