THE OPTIMIZATION OF ENZYMATIC SYNTHESIS FOR LAUROYL-N-METHYL GLUCAMIDE SUR FACT ANTS

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/29250/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12313
• Institution: Universitas Gadjah Mada

The optimization of enzymatic synthesis for lauroyl-N-methylglucamide surfactants is studied. The fraction of palm kernel oil namely lauric acid (AL) was amidificationed with N-methyl glucamine (MGL) to produce lauroyl-Nmethyl glucamide. Study was carriedout by using immobilizedlipase from Candidaantarctica (Novozyme 435 ® ), and tert-amylalcohol as a solvent. Response Surface Methodology (RSM) based on a five level, three variable design was employed, firstly, for studying the interactive effect of various parameters on the reaction, and secondly, for the optImization. The reaction parameters observed were Novozyme concentration, substrate molar ratio, and temperature. Simultaneouslyincreasing Novozyme concentration, substrate molar ratio, and temperature improves the reaction yield and the effect of temperature is noted more significant. The expected optimum condition was at molar ratio MGL: AL 1:1, the Novozyme concentrationof 8% and the reaction temperatureof 50-55°C. The reactions at the optimum condition produce the convertion of lauric acid of 64.5% and yield of 96.5%. With the optimization procedure the higher alkyl glucamide yield was achieved.