Study on Molecular: Genetic Diversity in D-Loop Mitochondrion DNA of Indigenous Java Duck (Magelang Duck)

• Main Author: Hartatik, Tety
• Format: Conference or Workshop Item NonPeerReviewed
• Language: English
• Published: 2012
• Subjects: Animal Cell and Molecular Biology; Animal Production
• Online Access: https://repository.ugm.ac.id/32353/1/19_2012_Purwantini_et_al_UNIV_ANDALAS.pdf; https://repository.ugm.ac.id/32353/
• Institution: Universitas Gadjah Mada

Individual duck in the population only inherited mitochondrial DNA from thefemale duck. Mitochondrial DNA had a higher rate of polymorphism than that ofnucleus DNA, mainly on Loop (D-Loop) which does not decode protein (non-coding region) with the highest polymorphism in mitochondrial genome. Polymorphism (diversity) of nucleotide order of D-Loop was often used to identify and analyze individual duck’s genetic diversity inter and intra population, phylogenesis and determining the genealogy inter family, channel or breed of the female. This research aimed to identify the genetic diversity based on nucleotide in D-loop mitochondrial DNA in the population of Magelang duck. The result gained could be used as the reference in determining the specific marker of mitochondrial DNA as the selection tool of Mallard duck conservation, breeding and propagation. The material was blood sample of Magelang ducks with eight different feather colors. Total DNA was isolated from the blood sample using DNA Isolation Kit (Geneaid). The amplification of D-Loop mtDNA with PCR technique used primary forwards DL-AnasPFand reverse DL-AnasPR. The PCR’s nucleotide produced was sequenced and analyzed. This research succeeded in (1) Amplifying PCR towards a 718 pb fragment in D-Loop mtDNA, (2) Determining the nucleotide order of Magelang duck with feather colors of 675 pb jarakan polos(plain brown), 707 pb bosokan(dark brown), 640 pb kalung ombo(brown with wide white feather around the neck), 661 pb gambiran(mixed brown and white), 644 pb jarakan kalung(brown with white necklace), 659 pb jowo polos(brown with specific pattern), 665 pb wiroko(mixed black and white), and 651 pb plain white(yellow bill and feet), (3) finding variant or morph of Magelang duck from eight different feather colors with the order of Mallard duck (Anas platyrhynchos)as many as 65, 83, 63, 41, 81, 63, 8 and 79 normal variant, respectively, in different positions. The conclusion drawn was the different order of nucleotide in D-Loop DNA mitochondria showed a diverse genetic characteristic in Magelang duck population. Key words: molecular, genetic diversity, D-Loop DNA mitochondria, Magelang duck