ISOLAT AKTIF DAUN Pseudocalymma alliceum (Lam.) Sandwith (BAWANG PUTIH ANGGUR) DAN AKTIVITAS ANTIVIRUSNYA TERHADAP VIRUS NEWCASTLE DISEASE

• Main Authors: , Nuning Rahmawati, , Dr. Ratna Asmah susidarti, MS., Apt.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/88321/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50621
• Institution: Universitas Gadjah Mada

Pseudocalymma alliaceum (Lam.) Sandwith has been reported to have many biological activities, such as anticancer, antifungal, antioxidan, hipolipidemia and antiviral against papaya mozaic virus. The objectives of this research were to obtain the active isolate from the leaf extract of this plant and to evaluate its antiviral activity against Newcastle Disease (ND) virus using embryonated chicken egg as culture medium. The extract was made by maceration. As much as 1 kg dried ground leaves were gradually macerated with petroleum ether, ethyl acetate and methanol. Evaporation of the solvents gave petroleum ether extract (4 g), ethyl acetate extract (33.5 g) and methanol extract (42 g). The extracts were assayed for their antiviral activities against Newcastle Disease (ND) virus. Hemagglutination method was used to count the virus titer. The percentages of ND viral growth inhibition of petroleum ether and ethyl acetate extracts were 80.95% and 61.90%, respectively. The methanol extract was found to be inactive. Separation of the ethyl acetate extract using vacuum liquid chromatography eluted by solvents of increasing polarity gave 21 fractions. Combining of these fractions based on their TLC profiles gave 8 combined fractions (A-H). Fraction F was the most active fraction which inhibited 87.50% of the virus growth. Fraction F was then washed with aceton to give white powder which was identified as ursolic acid based on its TLC profile and IR spectrum, compared to the authentic ursolic acid.