KADAR TESTOSTERON INTRATESTIKULAR Rattus novergicus STRAIN Sprague dawley AKIBAT PEMBERIAN KURKUMIN SETELAH STIMULASI hCG DENGAN ATAU TANPA PENAMBAHAN TEOFILIN Kajian in vivo untuk menentukan letak kerja kurkumin dalam transduksi sinyal steroidogenesis di sel Leydig

• Main Authors: , Husnul Khatimah, , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/88403/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50750
• Institution: Universitas Gadjah Mada

Curcumin is an active agent on Curcuma longa. Curcumin had so many biologic activities, one of them is anti fertility. Curcumin research in male reproductive system had been done. Curcumin reduced testis weight and serum testosterone. This study is done to find out site action of curcumin in signal transduction pathway steroidogenesis on Leydig cell and to know curcumin effect on testosterone intratesticular level in rats. This study was conducted to 27 male Sprague dawley rats aged 90 days. Rats divided into 9 groups treatment : group 1 rats were given aquades, group 2 rats were given a single dose of 50 IU hCG, group 3 rats were given CMC, group 4 rats were given curcumin, group 5 rats were given hCG and curcumin, group 6 rats were given theophylline, group 7 rats were given hCG and theophylline, group 8 rats were given theophylline and curcumin, group 9 rats were given hCG and theophylline and curcumin. Rats were sacrified on age 91 days. Testis was taken, homogenizer and sentrifuge. Supernatant was taken in order to measured intratesticular testosterone level. The result of this study show intratesticular testosterone level from 9 group treatment, each group are n = 3, are (1) 44.36±1.30 (aquades), (2) 41.53±16.44 (hCG), (3) 37.33±9.05 (CMC), (4) 54.16±29.82 (curcumin), (5) 49.36±5.05 (hCG + curcumin), (6) 44.20±22.09 (theophylline), (7) 83.23±18.81 (hCG + theophylline), (8) 44.73±22.90 (theophylline + curcumin), (9) 47.70±16.47 (hCG + theophylline + curcumin). Statistic analytic with ANOVA showed there is no significant difference (p>0.05). Statistic analytic with planned comparison with contrast showed there is significant difference on K7 with K2 and K9 group (p<0,05). This study have a conclusion that curcumin not decreased testosterone intratestikular level in rats and site action of curcumin located on before cAMP in signal transduction pathway steroidogenesis on Leydig cell .