EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN (Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2 melalui jalur cAMP)
Background: Several genes which their products are involved in the mechanism of ovulation are induced by the LH surge. One of them is COX-2, which one of molecular marker in mammals ovulation cycle. LH acts through protein kinase A (PKA) pathway in granulosa cells. PGV-0 has farmacologic action by i...
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[Yogyakarta] : Universitas Gadjah Mada
2011
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id-ugm-repo.885622014-08-20T02:54:36Z https://repository.ugm.ac.id/88562/ EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN (Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2 melalui jalur cAMP) , dr. RATNA DEWI PUSPITA , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D. ETD Background: Several genes which their products are involved in the mechanism of ovulation are induced by the LH surge. One of them is COX-2, which one of molecular marker in mammals ovulation cycle. LH acts through protein kinase A (PKA) pathway in granulosa cells. PGV-0 has farmacologic action by inhibiting prostaglandin biosynthesis in cyclooxygenase pathway. PGV-0 had action simillar with indometacin (selective COX-2 inhibitor) which cause follicle rupture in basolateral. Objectives: The objectives of this study is to investigate inhibition of PGV-0 to the expression of COX-2 in granulosa cells and to determine the action site of PGV-0 on COX-2 expression signal transduction in cAMP pathway. Methods: The subjects in this study were 27 female Rattus norvegicus strain Sprague Dawley. At the age of 28 days, subjects were induced by 5 IU PMSG. Rats were stimulated by LH 5 IU, then administered by theophylline 25 mg/kgBW orally, and PGV-0 55,2 mg/kgBW orally at 24 hours after PMSG administration. Aquades was administered as a control group. At the age of 30 days, all rats were sacrificed to make histology and immunohistology samples of the granulosa cells. Evaluation of COX-2 expression used immunohistochemistry technique with prime antibody of Rabbit Monoklonal (RB-9072-P) to determine the antigen location. Results: The PGV-0 group showed expression of COX-2 lower than the control group and LH+PGV-0 group. This supports that administration of PGV-0 decreased expression of COX-2 in granulosa cells. LH+teophylline+PGV-0 group showed expression of COX-2 not lower than LH+teophylline group, on the contrary this group showed higher expression of COX-2 than teophylline+PGV-0 group, supporting that the action site of PGV-0 is in the downstream cAMP of cAMP pathway and there were other pathways beside cAMP pathway stimulated by LH. Conclusions: The results suggest that PGV-0 inhibited expression of COX-2 in granulosa cells ovary follicle and the action site of PGV-0 is in downstream cAMP in cAMP pathway. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , dr. RATNA DEWI PUSPITA and , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D. (2011) EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN (Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2 melalui jalur cAMP). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50994 |
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ETD , dr. RATNA DEWI PUSPITA , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D. EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN (Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2 melalui jalur cAMP) |
description |
Background: Several genes which their products are involved in the mechanism
of ovulation are induced by the LH surge. One of them is COX-2, which one of
molecular marker in mammals ovulation cycle. LH acts through protein kinase A
(PKA) pathway in granulosa cells. PGV-0 has farmacologic action by inhibiting
prostaglandin biosynthesis in cyclooxygenase pathway. PGV-0 had action simillar
with indometacin (selective COX-2 inhibitor) which cause follicle rupture in
basolateral.
Objectives: The objectives of this study is to investigate inhibition of PGV-0 to
the expression of COX-2 in granulosa cells and to determine the action site of
PGV-0 on COX-2 expression signal transduction in cAMP pathway.
Methods: The subjects in this study were 27 female Rattus norvegicus strain
Sprague Dawley. At the age of 28 days, subjects were induced by 5 IU PMSG.
Rats were stimulated by LH 5 IU, then administered by theophylline 25 mg/kgBW
orally, and PGV-0 55,2 mg/kgBW orally at 24 hours after PMSG administration.
Aquades was administered as a control group. At the age of 30 days, all rats were
sacrificed to make histology and immunohistology samples of the granulosa cells.
Evaluation of COX-2 expression used immunohistochemistry technique with
prime antibody of Rabbit Monoklonal (RB-9072-P) to determine the antigen
location.
Results: The PGV-0 group showed expression of COX-2 lower than the control
group and LH+PGV-0 group. This supports that administration of PGV-0
decreased expression of COX-2 in granulosa cells. LH+teophylline+PGV-0 group
showed expression of COX-2 not lower than LH+teophylline group, on the
contrary this group showed higher expression of COX-2 than teophylline+PGV-0
group, supporting that the action site of PGV-0 is in the downstream cAMP of
cAMP pathway and there were other pathways beside cAMP pathway stimulated
by LH.
Conclusions: The results suggest that PGV-0 inhibited expression of COX-2 in
granulosa cells ovary follicle and the action site of PGV-0 is in downstream
cAMP in cAMP pathway. |
format |
Theses and Dissertations NonPeerReviewed |
author |
, dr. RATNA DEWI PUSPITA , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D. |
author_facet |
, dr. RATNA DEWI PUSPITA , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D. |
author_sort |
, dr. RATNA DEWI PUSPITA |
title |
EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM
AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH
DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN
(Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus
strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2
melalui jalur cAMP) |
title_short |
EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM
AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH
DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN
(Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus
strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2
melalui jalur cAMP) |
title_full |
EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM
AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH
DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN
(Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus
strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2
melalui jalur cAMP) |
title_fullStr |
EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM
AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH
DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN
(Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus
strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2
melalui jalur cAMP) |
title_full_unstemmed |
EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM
AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH
DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN
(Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus
strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2
melalui jalur cAMP) |
title_sort |
ekspresi cox-2 di sel granulosa folikel ovarium
akibat pemberian pgv-0 dengan stimulasi lh
dan dengan atau tanpa pemberian teofilin
(kajian in vivo untuk menentukan letak kerja pgv-0 pada rattus norvegicus
strain sprague dawley dalam transduksi sinyal ekspresi cox-2
melalui jalur camp) |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2011 |
url |
https://repository.ugm.ac.id/88562/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50994 |
_version_ |
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