EKSPRESI COX-2 DI SEL GRANULOSA FOLIKEL OVARIUM AKIBAT PEMBERIAN PGV-0 DENGAN STIMULASI LH DAN DENGAN ATAU TANPA PEMBERIAN TEOFILIN (Kajian in vivo untuk Menentukan Letak Kerja PGV-0 pada Rattus norvegicus strain Sprague Dawley dalam Transduksi Sinyal Ekspresi COX-2 melalui jalur cAMP)

• Main Authors: , dr. RATNA DEWI PUSPITA, , Prof. dr. Sri Kadarsih Soejono., M.Sc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/88562/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50994
• Institution: Universitas Gadjah Mada

Background: Several genes which their products are involved in the mechanism of ovulation are induced by the LH surge. One of them is COX-2, which one of molecular marker in mammals ovulation cycle. LH acts through protein kinase A (PKA) pathway in granulosa cells. PGV-0 has farmacologic action by inhibiting prostaglandin biosynthesis in cyclooxygenase pathway. PGV-0 had action simillar with indometacin (selective COX-2 inhibitor) which cause follicle rupture in basolateral. Objectives: The objectives of this study is to investigate inhibition of PGV-0 to the expression of COX-2 in granulosa cells and to determine the action site of PGV-0 on COX-2 expression signal transduction in cAMP pathway. Methods: The subjects in this study were 27 female Rattus norvegicus strain Sprague Dawley. At the age of 28 days, subjects were induced by 5 IU PMSG. Rats were stimulated by LH 5 IU, then administered by theophylline 25 mg/kgBW orally, and PGV-0 55,2 mg/kgBW orally at 24 hours after PMSG administration. Aquades was administered as a control group. At the age of 30 days, all rats were sacrificed to make histology and immunohistology samples of the granulosa cells. Evaluation of COX-2 expression used immunohistochemistry technique with prime antibody of Rabbit Monoklonal (RB-9072-P) to determine the antigen location. Results: The PGV-0 group showed expression of COX-2 lower than the control group and LH+PGV-0 group. This supports that administration of PGV-0 decreased expression of COX-2 in granulosa cells. LH+teophylline+PGV-0 group showed expression of COX-2 not lower than LH+teophylline group, on the contrary this group showed higher expression of COX-2 than teophylline+PGV-0 group, supporting that the action site of PGV-0 is in the downstream cAMP of cAMP pathway and there were other pathways beside cAMP pathway stimulated by LH. Conclusions: The results suggest that PGV-0 inhibited expression of COX-2 in granulosa cells ovary follicle and the action site of PGV-0 is in downstream cAMP in cAMP pathway.