POLIMORFISME GEN GROWTH HORMONE (GH) PADA SAPI MADURA DAN PERSILANGAN LIMOUSIN-MADURA
The present study was conducted to identify polymorphism of growth hormone (GH) gene in Madura and Madura crossed (Limousin-Madura) cattle, and its association with growth traits. Blood samples of 58 cattle were taken from population of Madura and Madura crossed, then all cattle are located in the s...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/89316/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=51749 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | The present study was conducted to identify polymorphism of growth hormone
(GH) gene in Madura and Madura crossed (Limousin-Madura) cattle, and its
association with growth traits. Blood samples of 58 cattle were taken from
population of Madura and Madura crossed, then all cattle are located in the same
area in Madura island. A 211 bp fragment of GH gene (spanning from the forth
intron region to fifth exon) was amplified using GHF (forward) primer:
5�GCTGCTCCTGAGGGCCCTTC-3�, and GHR (reverse):
5�CATGACCCTCAGGTACGTCTCCG-3�. Polymorphism was identified with
AluI restriction enzyme. The result indicated that two genotypes LL and LV were
found at the GH gene in population of Madura crossed cattle. The frequencies of
L and V alleles were 0.64 and 0.04, respectively. Between Madura and Madura
crossed cattle had a significantly differences in their body weight estimation, chest
girth, withers height, and body length. The appearance of LV genotype in
Limousin-Madura had no associated with body weight estimation also several
body measurement yet. |
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