POLIMORFISME C-106T GENA ALDOSA REDUKTASE (ALR) SEBAGAI FAKTOR RISIKO RETINOPATI DIABETIKA PADA PASIEN DIABETES MELLITUS TIPE 2 DI RSUP DR. SARDJITO YOGYAKARTA

• Main Authors: , JUJUK ANTON CAHYONO S.SI, , dr. Ahmad Hamim Sadewa, Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/89690/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52224
• Institution: Universitas Gadjah Mada

Background : Diabetes mellitus (DM) is a metabolic syndrome characterized by hyperglycemia and glucose intolerance, due to insulin resistance and insulin deficiency, or both. Retinopathy diabetics (RD) is a diabetes mellitus complication due to retinal abnormality, causing vision reduction, even blindness. Previous studies showed association between RD and aldose reductase C-106T gene polymorphism. This genetic polymorphism increases the level of sorbitol inside erythrocyte and pericyte in the retinal membrane leads to weakness of retinal capillary vessel and microaneurism. The aim of study : To know the presence of ALR C-106T gene polymorphism and frequency distribution among Javanese ethnic group in Dr. Sardjito Hospital Yogyakarta. To analyze the different of Erythrocytes osmotic fragility (EOF) among ALR genotypes and between DM type 2 with RD and without RD. To analyze ALR genetic polymorphism as a risk factor of DM type 2 with RD. Methods : Using case control study involved 40 patients with RD as case and 40 patients without RD as control groups. The C-106T ALR gene polymorphism was determined by PCR�RFLP method. EOF was analyzed by spectro. Genotype and allele distribution was analyzed using x 2 other data was analyzed using independent t-test and Mann-Whitney with p<0.05 considered as significantly different. Result : The genotype distribution among patients with RD were CC 33 (82.5%), CT 7 (17.5%) and among patients without RD were CC 27 (67.5%), CT 13 (32.5%). The genotype and allele distribution were not significantly different between two group (p=0.121 for genotype, p=0.151 for allele). Odds Ratio of genotype is 2.270 while allele is 2.023. EOF of CC genotype was higher that of CT genotype (p=0.047). Conclusion : The data showed not significant difference between CC and CT genotype distribution among type 2 DM patient with and without RD. EOF of CC genotype was higher that of CT genotype. C-106T gene polymorphism is a risk factor of retinopathy diabetics in type 2 DM.