PENGARUH EPIGALLOCATECHIN GALLATE TERHADAP VIABILITAS DAN PRODUKSI INTERLEUKIN 10 PADA SEL MONONUKLEAR DARAH TEPI PENDERITA PSORIASIS
Introduction: Psoriasis is a chronic recurrent inflammatory skin disease mediated by T cell lymphocytes and characterized with hyperproliferation of keratinocytes. Since there was no effective psoriatic modalities for preventing relapse and have minimal systemic side effects, psoriasis incidence ten...
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Main Authors: | , |
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Format: | Theses and Dissertations NonPeerReviewed |
Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
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Subjects: | |
Online Access: | https://repository.ugm.ac.id/89728/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=51907 |
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Institution: | Universitas Gadjah Mada |
Summary: | Introduction:
Psoriasis is a chronic recurrent inflammatory skin disease mediated by T cell
lymphocytes and characterized with hyperproliferation of keratinocytes. Since
there was no effective psoriatic modalities for preventing relapse and have minimal
systemic side effects, psoriasis incidence tend to increase twice in the last decade.
EGCG is a major green tea polyphenol that have antiproliferative effect on many
cancer cells also has a great impact on autoimmune and inflammatory disease
marked by abnormality of T cell such as psoriasis.
The aim of the study:
To know the effect of EGCG on the viability and IL-10 production of peripheral
mononuclear blood cells (PBMC) in psoriasis patients.
Material and Methods:
PBMC isolated from 5 psoriatics and healthy controls incubated for 48 hours in
RPMI medium containing of EGCG 50, 100, 200 µg/ml. The viability and IL-10
production were measured using MTT assay and IL-10 kits with ELISA reader.
Results:
The PBMC viability were decreased by EGCG both in psoriatics and healthy
controls significantly at 100 and 200 µg/ml (p<0.05).There was more descent
viability of healthy controls PBMC than psoriatic significantly at EGCG 100 µg/ml
(p=0.003). There was lower IL-10 production in psoriatic than healthy control
(p=0.012). The three doses of EGCG increased IL-10 production on psoriatic
(p=0.029) as for healthy control at 100µg/ml (p=0.001) dan 200 µg/ml (p=0.010).
Conclusions:
There were higher number of PBMC and lower level of IL-10 in psoriatics than in
healthy controls. EGCG decreased the viability and increased IL-10 production of
PBMCs in both psoriatics and healthy controls. |
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