EFEK EKSTRAK TUBER SARANG SEMUT (Myrmecodia tuberosa (non Jack) Bl.) TERHADAP PROFIL SEL TCD4+ DAN TCD8+ TIKUS SPRAGUE DAWLEY YANG DIINDUKSI DOKSORUBISIN
Doxorubicin (Dox) is an important anticancer drug. One of side effects known for Dox is immune-suppressing activity, which increases the risk of infection. Combination between Dox with immunomodulator herbs may reduce the side effects. This study aimed to explore effect of sarang semut tuber extract...
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Main Authors: | , |
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Format: | Theses and Dissertations NonPeerReviewed |
Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
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Subjects: | |
Online Access: | https://repository.ugm.ac.id/90191/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52541 |
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Institution: | Universitas Gadjah Mada |
Summary: | Doxorubicin (Dox) is an important anticancer drug. One of side effects
known for Dox is immune-suppressing activity, which increases the risk of
infection. Combination between Dox with immunomodulator herbs may reduce
the side effects. This study aimed to explore effect of sarang semut tuber extract
on TCD4+ and TCD8+ cell profiles of Dox-induced Sprague Dawley (SD) rats in
vivo. The study was done in two stages. The first stage was to explore the extract
and fractions of sarang semut tubers activities as immunomodulatory agents by an
in vitro method, and the second stage was to explore the effect of active extract on
TCD4+ and TCD8+ cell profiles on Dox-induced SD rats in vivo.
The study was started by macerating dried powder in ethanol 95%. The
solvent was evaporated and crude extract (EMT) was partitioned with equal
volume n-hexane:metanol 90% to yield purified extract (ETH) and n-hexane
fraction (FHE). ETH was further fractionated with ethyl acetate:water to yield
ethyl acetate fraction (FEA), and water fraction (FAI). The extract and fractions
were tested on phagocytosis activity of macrophage cells and proliferation of
lymphocyte cells by in vitro method. Total phenolic and flavonoid contents of
extract and fraction were determined to investigate the correlation to the
immunomodulatory activity. Active extract was further for tested its effect on
TCD4+ and TCD8+ cells profile on Dox-induced SD rats in vivo.
The result showed crude extract and fraction had promising activities on
immunomodulator in vitro. ETH at 20 µg/mL showed the highest activities on
phagocytosis by macrophage cells, while FEA at 100 µg/mL had the highest
activities on proliferation of lymphocyte cells. The highest total phenolic content
was EMT (3.75%) while FEA (2.15%) had flavonoid. In vivo assay showed that
ETH could maintain the number of TCD4+ cells, but unable to maintain the
number of TCD8+ cells. TLC profile of the active fraction, FEA showed the
presence of steroid/terpenoid, phenolic and flavonoid. As the conclusion, sarang
semut tuber extract is a potential immunomodulatory agent and may act as cochemotherapy in Dox use. |
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