ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS
Utilizing of chlorine-based chemicals in pulp bleaching process causes environmental pollution. Utilization of biological agents, such as enzymes for bleaching process is a wise measure to reduce the pollution. One of the enzymes that play an important role in pulp bleaching process is xylanase. Xyl...
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[Yogyakarta] : Universitas Gadjah Mada
2011
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id-ugm-repo.902622014-08-20T02:54:22Z https://repository.ugm.ac.id/90262/ ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS , YASINTHA MALLISA , Prof. Dr. Ir. Sebastian Margino ETD Utilizing of chlorine-based chemicals in pulp bleaching process causes environmental pollution. Utilization of biological agents, such as enzymes for bleaching process is a wise measure to reduce the pollution. One of the enzymes that play an important role in pulp bleaching process is xylanase. Xylanase facilitate bleaching by cleaves xylan that associated with lignin to release the lignin. The aims of this study are isolation and selection of soil fungus which have xylanolytic activity, identification selected isolate, optimation of xylanase production and its characterization. Isolation soil fungus from soil around the timber area and sawdust. The selection xylanolytic soil fungi based on the qualitative activity on solid medium and quantitave activity analyzing in liquid medium. Furthermore, selected fungi was identified based on the macroscopic and microscopic characteristics. Optimation of xylanase production included inoculums and xylan concentration, pH of medium and incubation time. Purification of xylanase was fractionated by ammonium sulfate precipitation and followed by gel filtration on sephadex G-75. Characterization of purified xylanase include molecular weight, investigate the effect of pH and temperature to their activity and measure of kinetic parameter (Michaelis constant (Km) and maximum velocity (vmax)). The result on the isolation step was obtained 108 fungal strain from soil. Of 108 isolates, 92 isolates were found positive for xylanase production which showed zone of hydrolysis on xylan-agar medium. Among these, 8 isolates were further selected for xylanase production by growing in liquid medium. The selection step resulted TKM-24 isolate as a selected strain which had highest xylanase activity (287,04 U/ml) and specific activity (3089,57 U/mg). TKM-24 isolate was identified as Aspergillus tamarii. Optimation of xylanase production was achieved using inoculums concentration 1 % (v/v), xylan concentration 1% (w/v), pH 5 and incubation time for 5 days. The specific activity increased after fractionation by ammonium sulfate and gel filtration chromathography, which achieved 23140,97 U/mg and 75671,88 U/mg, respectively. The molecular weight of the Aspergillus tamarii TKM-24 xylanase was estimated to be 25 kDa, showed optimal activity at 60 o C and pH 6. The Km and vmax were 24,4 mg/ml and 1520 µmol/ml/min for oat spelt xylan. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , YASINTHA MALLISA and , Prof. Dr. Ir. Sebastian Margino (2011) ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52794 |
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ETD , YASINTHA MALLISA , Prof. Dr. Ir. Sebastian Margino ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
description |
Utilizing of chlorine-based chemicals in pulp bleaching process causes
environmental pollution. Utilization of biological agents, such as enzymes for
bleaching process is a wise measure to reduce the pollution. One of the enzymes
that play an important role in pulp bleaching process is xylanase. Xylanase
facilitate bleaching by cleaves xylan that associated with lignin to release the
lignin. The aims of this study are isolation and selection of soil fungus which have
xylanolytic activity, identification selected isolate, optimation of xylanase
production and its characterization.
Isolation soil fungus from soil around the timber area and sawdust. The
selection xylanolytic soil fungi based on the qualitative activity on solid medium
and quantitave activity analyzing in liquid medium. Furthermore, selected fungi
was identified based on the macroscopic and microscopic characteristics.
Optimation of xylanase production included inoculums and xylan concentration,
pH of medium and incubation time. Purification of xylanase was fractionated by
ammonium sulfate precipitation and followed by gel filtration on sephadex G-75.
Characterization of purified xylanase include molecular weight, investigate the
effect of pH and temperature to their activity and measure of kinetic parameter
(Michaelis constant (Km) and maximum velocity (vmax)).
The result on the isolation step was obtained 108 fungal strain from soil.
Of 108 isolates, 92 isolates were found positive for xylanase production which
showed zone of hydrolysis on xylan-agar medium. Among these, 8 isolates were
further selected for xylanase production by growing in liquid medium. The
selection step resulted TKM-24 isolate as a selected strain which had highest
xylanase activity (287,04 U/ml) and specific activity (3089,57 U/mg). TKM-24
isolate was identified as Aspergillus tamarii. Optimation of xylanase production
was achieved using inoculums concentration 1 % (v/v), xylan concentration 1%
(w/v), pH 5 and incubation time for 5 days. The specific activity increased after
fractionation by ammonium sulfate and gel filtration chromathography, which
achieved 23140,97 U/mg and 75671,88 U/mg, respectively. The molecular weight
of the Aspergillus tamarii TKM-24 xylanase was estimated to be 25 kDa, showed
optimal activity at 60
o
C and pH 6. The Km and vmax were 24,4 mg/ml and 1520
µmol/ml/min for oat spelt xylan. |
format |
Theses and Dissertations NonPeerReviewed |
author |
, YASINTHA MALLISA , Prof. Dr. Ir. Sebastian Margino |
author_facet |
, YASINTHA MALLISA , Prof. Dr. Ir. Sebastian Margino |
author_sort |
, YASINTHA MALLISA |
title |
ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH
SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
title_short |
ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH
SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
title_full |
ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH
SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
title_fullStr |
ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH
SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
title_full_unstemmed |
ISOLASI DAN KARAKTERISASI XILANASE JAMUR TANAH
SEBAGAI AGENSIA PEMUTIH BUBUR KERTAS |
title_sort |
isolasi dan karakterisasi xilanase jamur tanah
sebagai agensia pemutih bubur kertas |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2011 |
url |
https://repository.ugm.ac.id/90262/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52794 |
_version_ |
1681228932035117056 |