EFEKTIVITAS BAWANG PUTIH (Allium sativum L.) TERHADAP INFEKSI Pseudomonas anguilliseptica PADA IKAN MAS (Cyprinus carpio)

• Main Authors: , MUHAMAD ARFAN LESMANA, , Prof. drh. Kurniasih, MV.Sc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/90602/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52825
• Institution: Universitas Gadjah Mada

Red spot disease is a disease that causes high mortality in fish and has spread to many regions in Indonesia. The disease caused by Pseudomonas anguilliseptica, this bacteria has the highest pathogenecity compared to other Pseudomonas in fish.The prevalence of bacteriaresistantto antibioticshas become amajor problem in fish farming, therefore the prevention study using natural ingredients is necessary to avoid antibiotic residues. The aim of this study is to find out the effectiveness of the use garlic extract for the prevention of infection red spot infection disease and detection of Pseudomonas anguilliseptica infections in common carp molecularly (PCR). Pseudomonas isolates that used in this study is a collection of Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. Fish were divided into 4 groups, group 1 as negative control (standard feed), group 2 as positive control (standard feed), group 3 were fed using garlic extract 5% and group 4 were fed using garlic extract 10%. Fish groups 3 and 4 were fed using garlic extract for 1 month. Fish groups 2, 3, and 4 infected using 0.1 ml LC50 suspension of P. anguilliseptica intramuscularly on day-31, fish group 1 injected with 0.1 ml physiological saline intramuscularly on day-31. Blood test (number of heterophile and lymphocyte) and the increase of body weight on day-7 post infection. Reisolation bacteria and analysis PCR of P. anguilliseptica from liver and kidney tissues conducted on day 14 post infection. The final results showed that garlic extract 5% are able to increase non-spesific immunity significantly in common carp (p<0,05), feeding garlic extract 5% dan 10% can improve the survival rate of common carp against P. anguilliseptica infections, feeding garlic extract 10% can increase body weight significantly (p<0,05) in common carp, isolation and identification of P. anguilliseptica from the liver and kidney tissues on day-14 post infection showed negative results and genotipically the bacteria not present in the liver and kidneys tissues on day-14 post infection.