ANATOMI AKAR, BATANG, DAUN DAN KANDUNGAN GIZI TANAMAN MELON (Cucumis melo L.) KULTIVAR MELODI GAMA-1 HASIL POLIPLOIDISASI DENGAN BIO-CATHARANTINE

• Main Authors: , YUNIASIH, S.Pd, , Dr. Maryani, M. Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/90692/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53021
• Institution: Universitas Gadjah Mada

Polyploidization produces plants with larger roots, stems, leaves, flowers and fruit than diploid plants. This study aims to determine the anatomy of roots, stems, leaves and plant nutrient content of melon cultivar Melody Gama-1 produced by polyploidization with bio-catharantine. This study used polyploid and non polyploid melon cultivars Melody Gama-1 produced by induction using ethanolic leaf extract of periwinkle leaf (Catharanthus roseus (L) G. Don,) with a concentration of 0.05%. Anatomical observations were done by making cross section of roots, stems and leaves using the paraffin method. The anatomical of roots are the root diameter, the cortex thicknes, the bundle sheat thicknes, and the diameter of the xylem. The anatomical of stems are stem diameter, the epidermis thicknes, cortex thicknes, the bundle sheat thicknes, and diameter of the xylem. The anatomical of leaves are thicknes of the midrib, the bundle sheat thicknes, palisade parenchyma thicknes, spongy parenchyma thickness, xylem diameter, stomata size, number and index of stomata, and chlorophyll content. Nutrients content included protein, minerals (Ca, P, Fe), vitamin C and water content. Observation of the size and distribution of stomata were preparing epidermal leaves using leaf clearing. Chlorophyll content was determined using Harbone methods, the protein content was measured using the Kjeldahl method, the mineral content was measured using the spectrophotometer method, vitamin C test with iodine titration. Data were analyzed descriptively and quantitatively. Quantitative data were analyzed using t test with significant level of 5%. The results showed polyploid plants have a larger size of organs included plant height, root length, length and leaf area than control. Anatomically polyploid plant have larger cell, seen in cortex, epidermis, and xylem. Stomata poplyploid longer, but the number of stomata fewer and smaller indexes. Chlorophyll, protein, phosphorus, vitamin C content increased, but the content of Ca, Fe, and the water decreases.