IDENTIFIKASI GOLONGAN SENYAWA PENURUN KADAR GLUKOSA DARAH TIKUS PUTIH (Rattus norvegicus Berkenhout, 1769) HIPERGLIKEMIA PADA DAUN SUKUN (Artocarpus altilis (Park.) Fosberg.)

• Main Authors: , NUBLAH,SP, , Prof. Sukarti Moeljopawiro, Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/90697/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53007
• Institution: Universitas Gadjah Mada

Sukun (Artocarpus altilis (Park.) Fosberg.) leaf has been extensively used to treat various health problems including diabetes mellitus. . The scientific studies of hypoglycemic activity of breadfruit leaves are relatively very few. Therefore the objective of this research was to identify the bioactive compound that have activity to lower blood glucose. Forty hyperglycemia rats, male Wistar 2 month old were used in this study. Hyperglycemia rats were made by injecting glucose monohydrate 1,35 g/kg body weight. For extract efectivity test, 20 rats were divided into 4 groups of 5. One group as a control, the next two groups were given extract of chloroform and methanol respectively, and the last group were given boiled water extract of sukun leaves. The best extract was fractionated by partition using ethyl acetate. Another 20 rats were used to analyze the activity of the fraction. They were again divided into 4 groups of 5. One group as a control, another one group were given glibenclamide as a reference, and another two groups were given 2 kinds of fraction (water fraction and ethyl acetate fraction). The dose that used for each extract, boiled water extract of sukun leaves, and fraction of the best extract were at 150 mg/kg body weight. Blood glucose was determined at minute 0, 60, 120, 180, 240, and 300. Ethyl acetate fraction of boiled water extract was analyzed using Thin Layer Chromatography followed by application of various detection reagents for identification of compounds.. ANOVA was used to analyze the difference among samples followed by LSD 5% to locate the difference. The results showed that boiled water extract of sukun leaves has the highest activity, followed by chloroform extract. Both ethyl acetate and water fraction of boiled water extract of sukun leaves could lower the blood glucose, but were not as good as glibenclamide. The bioactive compound groups that have hypoglycemic activity were classified as phenolic, flavonoid, alkaloid and terpenoid. It could be concluded that traditional used of sukun leaf boiled water to treat diabetes mellitus has been already proper way.