Genetic Variability in Geographical Populations Of Culex quinquefasciatus SAY (Diptera: Culicidae) F rom Lymphatic Filariasis Endemic Areas Based on Random Amplified Polymorphic DNA Analysis

• Main Authors: Astuti, Rr. Upiek Ngesti Wibawaning, Handayani, Niken Satuti Nur, Hadisusanto, Suwarno, Poerwanto, Soenarwan Hery
• Format: Conference or Workshop Item PeerReviewed
• Language: English
• Published: 2012
• Subjects: Zoology
• Online Access: https://repository.ugm.ac.id/91950/1/Genetic%20Variability%20in%20Geographical%20Populations%20Of%20Culex%20quinquefasciatus%20SAY%20%28Diptera%20Culicidae%29%20From%20Lymphatic%20Filariasis%20Endemic%20Areas%20Based%20on%20Random%20Amplified%20Polymorphic%20DNA%20Analysis.pdf; https://repository.ugm.ac.id/91950/
• Institution: Universitas Gadjah Mada

Lymphatic filariasis is a serious public health problem in llie world including in Indonesia. The number of endemic areas increased with high incident and chronic patients. In urban areas, the disease is caused by filarial nematodes, called Wucheria bancrofi, and is spread through the bite of infected mosquitoes, especially the species of'Culex quinquefasciatus Say. Host-parasite relationship remains unknown, as well as the geographical differences of the vector. The objective of this research was to determine the genetic variability of mosquitoe vector Cx. quinquefasciatus from two filariasis endemic areas, Pekalongan District. Mosquitoes were collected indoors and outdoors by using "man landing biting" method andsweapnet. The DNA extraction from each single female mosquito was performed, and then the DNA was used as a P G R template with oligonucleotide RAPD primer OPA 11. The PCR products were analyzedby electrophoresis in 1% (w/v) agarose gel . The result showed 15 loci with 100% polymorphism. Keywords: genetic variability , RAPD, Culex quinquefasciatus, ilymhatic filariasis