IDENTIFIKASI STRUKTUR SENYAWATOKSIK TERHADAP SEL KANKER PAYUDARA DAN RAHIM DALAM EKSTRAK KLOROFORM BUAH MERAH (Pandanus conoideus Lamk.) DAN KAPANG KONTAMINAN PENGHASILAFLATOKSIN

• Main Author: , Sukarti Moeljopawiro
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Lembaga Penelitian dan Pengabdian Kepada Masyaraka 2009
• Online Access: https://repository.ugm.ac.id/95223/; http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3041
• Institution: Universitas Gadjah Mada

<p>A number of traditions came to dominate the practice of herbal medicine in the western world at the end of the twentieth century. Recently, buah merah have been extensively used to treat cancer. It was found that the best extract that had the highest toxicity on breast cancer ana cervix cancer cells was chloroform extract of Maler. But the kind of compounds on those extracts has not been identified. Therefore, the objectives of this study were to identify the structure of toxic compounds on breast and cervix cancer cells and the aflatoxins production of mold contaminants.<br /> Buah merah local variety Maler, cancer cell line T47D and HeLa were used in this study. Doxorubicin was used as a reference in cytotoxicity assay. Buah merah was extracted using chloroform.The extract was fractionated using vacuum column chromatography followed by TLC. Similar profiles of fractions were combined. Cytotoxicity assay was performed using MTT assay. The IC value was determined using linear regression analysis. The most toxic fraction was subjected to preparative TLC followed by MTTassay. The most toxic isolate was analyzed using TLCfollowed by application of various detection reagents for identification of the compounds. The most toxic isolate was fractionated using the same method followed by MTT assay using Doxorubicin as a reference. The structure of those compounds was elucidated using spectroscopy method (UV, IR and MS). The aflatoxins production of mold contaminants was detected qualitatively using in vitro analysis and quantitatively using TLC scanner.<br /> Results of MTTassay of isolates obtained from preparative TLC showed that the best isolate both on T47D and HeLa were the one in the middle position. After isolate fractionation followed by MTT assay, IC50 of the most toxic fraction on T47D (41.64 g/ml) was lower than that of Doxorubicin (45.32 ~g/ml). Whereas the most toxic fraction on HeLa did not show any anti cancer activity (IC50 171.98 gI ml), therefore this toxic fraction structure was not analyzed. Analysis of the toxic compounds groups both on T47D and HeLa cells revealed that the toxic compounds groups were terpene.<br /> Results of structure elucidation of the toxic compounds on T470 cells by spectroscopy UV showed that the compounds did not have chromophore groups and conjugated bonds. Spectra IR revealed that the compounds were long chain compounds with carboxylic group. Spectra MS showed that the toxic compounds were hexadecanoic acid and 9-0ctadecenoic acid (Z). Identification of mold contaminats from fresh fruit and chloroform extract of Maler showed that only 2 mold isolates producing aflatoxins were found qualitatively, while detection quantitatively the aflatoxins were not detected.<br /> It could be conduded that the toxic compounds of chloroform extract of Maler on breast and cervix cancer cells were terpene. The structures of those compounds were hexadecanoic acid and 9-octadecenoic acid (Z). Molds producing aflatoxins were not present on fresh fruit and chloroform extract of buah merah local vanety Maler, therefore they were safe to be consumed.</p>