EFEK EKSTRAK DAUN TEH (Camellia sinensis (L.) Kuntze) TERHADAP GAMBARAN HEMATOLOGI, KADAR NO, AKTIVITAS GPx PADA INDIVIDU DENGAN POLIMORFISME GENA NOS3 DAN &#948

• Main Author: , Hernayanti
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Fakultas Biologi Universitas Gadjah Mada 2012
• Online Access: https://repository.ugm.ac.id/95363/; http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3181
• Institution: Universitas Gadjah Mada

<p>Lead have used as ceramics, fireproof materials and additive agent gasoline. Lead is toxic to human especially for lead-exposed individual. Green tea been extensively used to treat many kind of disease because its high contain antioxidant called catechin. The objective of these studies were to know the effect green tea as a chelating agent of Pb. This research was conducted in two stages. The first stage was to know the best green tea formulation for treatment using Wistar Fourty two male Wistar rats were used in this studies. Rats were devided into groups of 6. First group as a negative control. The rest were the treatment groups that were given Pb acetate orally for 66 day. On the 35 th day of studies group II, III, and V were given leaf green tea extract at 0 g/kg/bw/day (positive control), 0.75 g/kg/bw/day, 1.5 g/kg/bw/day, 3 g/kg/bw/days respectively. The group VI was given green tea infusion 5 g/kg/bodyweight/days and the group VII was given dimercaprol 3 g/kg/bw/days, as a standard clinical medicine. Parameter recorded were hematological profile that measured by hematology analyzer and blood lead, malondialdehyde, glutathione peroxidase activity, nitric oxide that measured commercial kits. The different among treatments were analyzed using ANOVA, followed by Duncan to locate the different. The supporting parameters histopathological liver and kidney of rat, was observed on 66 th day, urine Pb levels and body weight of rats was observed on 0 and 66 th day.<br /> These results showed that treatment of all doses of green tea extract, green infusion and dimercaprol could decrease Pb and MDA level and increase NO level and GPx activity. The groups of rats that treated with green tea solution dose g/kg/bw/day, gave a better histological figure compare with all of rats group. Based on these results, green tea infusion dose 5 g/kg/bw/day is the best treatment. The second stage of studies using a survey method with a cohort design These subject were 30 autorepair workers as cases subject and 30 control subject come from non polluted area (Pamijen village). The genotype individu polymorphisms NOS3 and ALAD gene were analyzed by Polymerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphisms (RFLP) with MboI restricted enzyme for NOS3, MspI restricted enzyme for ALAD gene. Parameter recorded were hematologic profile, blood lead level, malondialdehyde level, glutathione peroxidase activity and nitric oxide level. Suppporting parameters include systolic and dyastolic blood preassure. Data were analyzed by pair t-test. <br /> These results showed that 40% of cases subject were detected as polymorphisms individual of gene NOS3, with GA genotype. As manya as 60% of cases subject were detected as non polymorphisms individual with GG genotype. One hundred percent of control subject detected as non polymorphisms individual of NOS3 and ALAD gene. Thirty percent of 30 cases subject were individual of ALAD gene polymorphisms, while 70% of cases subject, detected as non polymorphisms of ALAD gene. Thirty days after they were given green tea infusion their Pb level, MDA level, systolic and dyastolic blood pressure decreased but their NO level, GPx activity, Hb level and erythrocyte increased.<br /> It could be concluded that green tea could improve hematologic profile, histologic figure of liver and kidney and Pb level on rat-exposed lead. Green tea could improve hematologic profile on lead-exposed individual with &delta