Analysis of expression of anti-microbial peptides and E-cadherin in radicular cysts and established dental epithelial cells

• Main Author: , Kiyohide Ishihata
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Fak.Kedokteran Gigi Universitas Gadjah Mada 2012
• Online Access: https://repository.ugm.ac.id/96129/; http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3947
• Institution: Universitas Gadjah Mada

<p>Background: Radicular cysts are inflammatory cysts that develop in association with an infected root canal. It is thought that persistent inflammation may stimulate proliferation of dental epithelial cells around the root to form a cyst. However, pathogenesis and the mechanism of epithelial proliferation have not been clarified. Recently, antimicrobial peptides (AMPs) were identified from jaw cyst fluid, and may participate in inflammatory responses to bacterial infection and play an important role in defense mechanisms. The expression of AMPs in radicular cysts and the response of dental epithelial cells to bacterial infection were examined in the present study. Methods: Twenty samples of radicular cysts and apical granulomas were assessed for the expression of alpha-defensin (HNP), cathelicidin LL37, beta-defensinl-2 (hBDI-2), and E-cadherin by immunohistochemistry. An immortalized rat dental epithelial cell line (HAT-7) was used for in vitro experiments and assayed for E-cadherin and-AMP production after incubation with/without lipopolysaccharide (LPS) by quantitative real-time polymerase chain reaction. Cell proliferation after incubation with/without LPS or LL37 was assayed by a methyl thiazolyl tetrazolium (MTT) assay. Results.: HNP was observed in apical granulomas, while HNP and hBD2 were observed in radicular cysts. E-cadherin was observed more in radicular cysts than apical granulomas. LPS stimulation facilitated clear hBD2 and E-cadherin transcription and protein induction in HAT7. Cell proliferation was not enhanced after LPS stimulation. Conclusion: Dental epithelial cells produce AMPs and consolidate epithelial intercellular junctions against bacterial infection and may playa role as a secondary barrier for preventing infection.</p>