VARIASI GENETIK PISANG TONGKAT LANGIT (Musa troglodytarum L.) BERDASARKAN KARAKTER MORFOLOGIS DAN MOLEKULAR
Fei banana (Musa troglodytarum L.) is one of banana species which is grown only in East of Indonesia, mainly in Moluccas and Papua. In Moluccas, distribution of fei banana is limited to Ambon, Haruku, Saparua, Nusalaut, and Seram islands. This banana has a unique type because it has bunches growing...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/97311/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53070 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Fei banana (Musa troglodytarum L.) is one of banana species which is
grown only in East of Indonesia, mainly in Moluccas and Papua. In Moluccas,
distribution of fei banana is limited to Ambon, Haruku, Saparua, Nusalaut, and
Seram islands. This banana has a unique type because it has bunches growing up.
The fruit of this banana is edible and has a potential as raw material of drug.
Recently, banana in Moluccas has been developing as alternative food-stuff of
rice substitute. The development of potency and production of banana as source of
germ plasm conservation is needed by excellent variety geneticaly. This research
was aimed to analyze genetic variation of fei banana between population based on
morphological and molecular characters.
Exploration was conducted in 5 islands in Moluccas based on
morphological character. Moreover, DNA was isolated from leaf and amplified by
Polymeration Chain Reaction (PCR) with 12 primers Random Amplified
Polymorphic DNA (RAPD). Morphology data and PCR product was assembled
into a binary data matrix, followed by similarity, clustering, comparation analysis
using Unweighted Pair-Group Methods Using Arithmatic Averages (UPGMA)
method and NTSys-PC program. Calculation percentage of monomorphism and
polymorphism bands in all population for detection of genetic variation.
The results showed that there was 8 location on five populations of fei
banana which explorated and had various characters. Based on scoring results
from 12 primers RAPD there were 128 DNA bands which consisted of 101
polymorphic DNA bands and 27 monomorphic DNA bands. Analysis
comparation showed that miscompatible of genetic variation based on
morphological and molecular characters. |
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