DETEKSI PENYEBAB PENYAKIT KERUPUK DAN MOSAIK PADA TEMBAKAU DAN PENGENDALIAN KUTU KEBUL DENGAN BARRIER SYSTEM DI PTPN X (PERSERO), KLATEN

• Main Authors: , TRI MARUTO AJI, , Dr. Ir. Sedyo Hartono, M.P
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2012
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/98705/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55471
• Institution: Universitas Gadjah Mada

Since 2009, the leaf curl disease symptom was observed on tobacco plants under net shadow of PTPN X (Persero) and has caused yield losses up to 70%. The disease was likely to be associated with the existence of high population of the sweet potato-whitefly (Bemisia tabaci) and the symptoms resembled to that reported for Begomovirus infection on eggplant and tomatoes. This study aimed to identify the pathogen and how to control the disease using of physical barriers in combination with a biological barrier to avoid B. tabaci enter the farm. The research was done by surveying tobacco diseases to know the diseases intensity and to collect samples for further laboratory research. To identify the pathogen caused the disease, total DNA was extracted from leaf curl symptom of tobacco, PCR analyses using three primers set TYLCV 1840F&IL2642R, Krusty&Hommer, and Pal1v1978 and Par1c496, the universal primers for Begomovirus. PCR results showed a single DNA band with approx. size of 800 bp when DNA was amplified by PCR using TYLCV 1840F&IL2642R primers, and two DNA bands of approx. size 580bp and 650 bp were obtained using Krusty & Hommer primers, and a 1600 bp DNA band amplified by PCR using TYLCV 1840F&IL2642R. Viral identification using primers TobRT-up1 and TobRT-Do2 for Tobamoviruses revealed a single DNA band with approx. size of 568 bp when cDNA from mosaic symptoms of tobacco was used as a template for PCR. One DNA product of Begomovirus sample was then directly sequenced. Nucleotide sequence analysis showed that leaf curl disease of tobacco is caused by Tomato yellow leaf curl virus (TYLCV), a member of the Genus Begomovirus, the Family Geminiviridae. BLAST searched revealed a highest DNA homology to TYLCV-Kan 1 and TYLCV-Kan 2 isolates of Thailand. Research for management of insect vectors B. tabaci were done using three methods: (1) Physical barrier using net barrier