EFEK SITOTOKSIK, PROLIFERASI DAN APOPTOSIS FRAKSI AKTIF AKAR TUMBUHAN MEKAI (Albertisia papuana Becc.) TERHADAP SEL KANKER PAYUDARA (T47D)

• Main Authors: , WIDYASARI, , Prof. Dr. Istriyati, MS.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2012
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/99115/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55522
• Institution: Universitas Gadjah Mada

Medicinal plants of East Kalimantan needs to be explored because of high value. One of these medicinal plants are mekai that usually use by the Dayak community for the treatment of various degenerative diseases such as hypertension, stroke and cancer. The commonly used for cancer treatment is the root of mekai, the scientific studies of the effect of mekai on the growth of cancer cells has not been done. Therefore the objectives of this study was to determine the cytotoxic effect of mekai root on breast cancer cells (T47D). The roots of mekai plant were extracted gradually using chloroform and methanol, and boiled with water. All extracts were tested on breast cancer cells (T47D) by MTT Assay method. The most toxic extract was fractioned by Vaccum Liquid Chromatography (VLC) and followed by Thin Layer Chromatography (TLC). The similar TLC profiles were combined. The toxicity of the combined fraction were tested on the T47D cells again by the MTT Assay. And the effect of the proliferation of the most toxic fraction were done by doubling time assay and the apoptosis test were done by double staining method. The most toxic fraction was also analyzed using TLC followed by the application of various detection reagents for identification of the active compound. Test cytotoxicity by MTT assay method showed that the chloroform extract of the mekai roots had the highest cytotoxic effect on T47D cells (IC-50 4.418 ug/ml), followed by methanol extract (IC-50 37.892 ug/ml) and water decoction mekai roots (IC-50 113.207 ug/ml). Cytotoxicity test showed that the combined fraction of chloroform extract fraction III had the highest cytotoxic effect against breast cancer cells (T47D), IC- 50 = 0,913 ug/ml. And these fraction also inhibit proliferation and induced apoptosis of T47D cells. The active compound in those fraction was alkaloid. It could be concluded that mekai root extract could inhibit proliferation and increased apoptosis of breast cancer cell (T47D), the active compound in mekai root was alkaloid.