POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO
Signal transduction disregulation, i.e. MAP kinase pathway leads to degenerative disease development, including cancer. Today, people are seeking for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness and decrease its side effect. Curcumin is a potential chemopreventive a...
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[Yogyakarta] : Universitas Gadjah Mada
2012
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id-ugm-repo.998552016-03-04T08:46:32Z https://repository.ugm.ac.id/99855/ POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., ETD Signal transduction disregulation, i.e. MAP kinase pathway leads to degenerative disease development, including cancer. Today, people are seeking for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness and decrease its side effect. Curcumin is a potential chemopreventive agent targeting kinase proteins on their ATP binding site. Thus, Faculty of Pharmacy UGM developed curcumin analogue, PGV-0. Due to its insolubility, Na PGV-0 was then synthesized. This research was done to study Na PGV-0 potential as cochemotherapeutic agent targeting proteins involved in MAP kinase pathway, especially EGFR and IKK, and its effector protein, Pgp and COX-2. Co-chemotherapeutic activity was determined by cytotoxicity single and in combination with doxorubicin on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr cells using MTT assay. While the molecular mechanisms were explored in silico using PLANTS software. Na PGV-0 as ligand was prepared using Marvin Sketch, while protein targets were prepared using YASARA. Na PGV-0 exhibited cytotoxic property on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr cells with IC50 values of 52.33 + 6.36 [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Endah Puspitasari and , Dr. Agung Endro Nugroho, M.Si., Apt., (2012) POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56219 |
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ETD , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO |
| description |
Signal transduction disregulation, i.e. MAP kinase pathway leads to
degenerative disease development, including cancer. Today, people are seeking
for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness
and decrease its side effect. Curcumin is a potential chemopreventive agent
targeting kinase proteins on their ATP binding site. Thus, Faculty of Pharmacy
UGM developed curcumin analogue, PGV-0. Due to its insolubility, Na PGV-0
was then synthesized. This research was done to study Na PGV-0 potential as cochemotherapeutic
agent targeting proteins involved in MAP kinase pathway,
especially EGFR and IKK, and its effector protein, Pgp and COX-2.
Co-chemotherapeutic activity was determined by cytotoxicity single and in
combination with doxorubicin on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr
cells using MTT assay. While the molecular mechanisms were explored in silico
using PLANTS software. Na PGV-0 as ligand was prepared using Marvin Sketch,
while protein targets were prepared using YASARA.
Na PGV-0 exhibited cytotoxic property on MCF-7 ori, MCF-7/DOX,
HeLa, and WiDr cells with IC50 values of 52.33 + 6.36 |
| format |
Theses and Dissertations NonPeerReviewed |
| author |
, Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., |
| author_facet |
, Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., |
| author_sort |
, Endah Puspitasari |
| title |
POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_short |
POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_full |
POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_fullStr |
POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_full_unstemmed |
POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_sort |
potensi ko-kemoterapi na pgv-0 dengan doxorubicin
terhadap berbagai sel kanker
dan penelusuran target molekulernya
pada jalur map kinase:
pendekatan in vitro dan in silico |
| publisher |
[Yogyakarta] : Universitas Gadjah Mada |
| publishDate |
2012 |
| url |
https://repository.ugm.ac.id/99855/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56219 |
| _version_ |
1681230624186171392 |