PENGARUH PEMBERIAN RECOMBINANT HUMAN ERYTHROPOIETIN TERHADAP KEKUATAN TARIK KULIT PASCA INSISI DENGAN PERDARAHAN AKUT (Penelitian Eksperimental pada Tikus Wistar)

• Main Authors: , Daniel Tumbur Hasintongan H, , drg. Prihartiningsih, SU, SpBM (K)
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2012
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/99950/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56354
• Institution: Universitas Gadjah Mada

Wound healing process in skin is a response of an injured body part. Tissue oxigenation influences the wound healing. Acute bleeding 30% of total human blood causes haemoglobin level reduction. A low level of haemoglobin can decrease the wound oxigenation thus disrupts the wound healing. Recombinant human erythropoietin is a hormone that induces the hematopoiesis. Furthermore, this hormone can increase angiogenesis in wound healing. The purpose of this research is to get an information about the influence of recombinant human erythropoietin application to wound healing in skin after the incision with acute bleeding by measuring the skin tensile strength. Pure experimental research is done on 24 wistar rat, divided into 3 groups, respectively 8 rat. First the bleeding level is made into 30% on 16 rat randomly thus get a low haemoglobin level. Group A is undergone a bleeding then was made an incision on their skin. Group B is undergone the bleeding then was made an incision on skin then given a recombinant human eryhtropoietin injection 500 IU/kg for 7 days. Group C as countrol group which is not get the bleeding then is having an incision on skin. All groups is taken care and given a standard food and drink for 7 days. On the 7 th day is having a euthanasia then the skin is taken and sliced by its pattern. Tensile strenth test is being done on the skin. The data from the skin tensile strength is being analized by Kruskall Wallis test and post hoc Mann-Whietney test to see the difference between these groups. The result of this research by using the Kruskall Wallis test shows a significant difference with the skin tensile strength on Group A, B, and C (P>0,05). In post hoc Mann-Whietney test there is a significant diffference between Group A and B, Group A and C (P>0,05), meanwhile there is no significant difference between Group B and C (P<0,05). The conclusion is that the application of recombinant human erythropoietin can increase rat skin tensile strength after an incision with acute bleeding.