EKSPRESI P53 DAN Ki 67 PADA DIFFUSE LARGE B CELL LYMPHOMA TIPE NODAL DAN EKSTRANODA

• Main Authors: , Rodlijatul Machfudzoh, , dr. Indrawati, SpPA(K)
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2012
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/99965/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56375
• Institution: Universitas Gadjah Mada

Background: Diffuse Large B cell Lymphoma (DLBCL) is the most common malignant tumor of lymphoid tissue derived from the mature B cells. Based on the location, DLBCL can be divided into nodal and extranodal DLBCL, and they proposed differently to have the clinical behaviors and prognostic. They are caused by many factors including because of the proliferation and apoptosis of tumor cells. The markers of proliferation and apoptosis are used the proteins of P53 and KI-67, and the expressions of P53 and KI-67 can be used to distinguish the aggressiveness of nodal and extranodal DLBCL. Various studies on the expressions of P53 and KI-67 DLBCL showed controversial results. Objectives: To investigate the difference mean of P53 and Ki67 protein expressions between nodal and extranodal DLBCL. Methods: This study was a retrospective cross sectional. The total sample size of 40 DLBCL samples were taken from Sardjito�s Hospital at Yogyakarta that consist of 21 nodal DLBCL and 19 extranodal DLBCL samples respectively. The preparation was stained using immunohistochemistry with monoclonal P53 and KI-67antibody. The expression of P53 and Ki-67 was calculated from the number of positive cell nuclei are stained brown. The differences mean of the P53 and Ki67 DLBCL of nodal and extranodal were analyzed using Independent Sample t-Test. Results: In this study find no significant difference between age clinical characteristics and gender to nodal and extranodal locations (p = 0.527, p = 0.1000). This study also find not significant differences mean in P53 expression to nodal and extranodal DLBCL (p = 0.778), but the mean of Ki-67 expression to nodal and extranodal DLBCL significant difference (p = 0.04). Conclusion: The study find not significant differences between nodal and extranodal DLBCL in terms of age, gender and P53 expression, but they significant difference in Ki67 expression. The mean of Ki-67 nodal expression is higher than the mean of Ki-67extranodal expression which indicate that nodal DLBCL more aggressive than extranodal DLBCL.