Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum

• Main Author: Lam, Chi Yan
• Format: Thesis
• Language: English
• Published: 2015
• Subjects: TP Chemical technology
• Online Access: http://eprints.intimal.edu.my/427/1/BBTEI%20-%2069.pdf; http://eprints.intimal.edu.my/427/
• Institution: INTI International University

DNA barcodes are used to assign samples of unknown origin to specific taxonomic groups i.e., short DNA sequences (600-800bp) of a standard segment for species identification. In my study, I used the matK and rbcL barcode sequences to identify the Jasminum grandiflorum, Jasminum sambac and Jasminum nitidum. In these species, Edwards’s method was able to provide of sufficient quality and quantity of DNA for PCR. In my results, the matK gene showed ease of amplification compared to rbcL gene. The second trial of marK with diluted DNA samples show higher success rate. For species discrimination, the neighbor-joining tree was used to construct phylogenetic trees based on T92 model as it was the best lit sequence model based on mwK and rbcL gene. Based on the maiK gene, J, grandiflorum is more closely related to J. sambac (Distance= 0.010) then J. nitidum (Distance 0.020); while based on the rbcL gene. J. sambac is more closely related to J. nitidum (Distance= 0.007) then J. grandiflorum (Distance= 0.018). Although the tree topology based on maiK and rbcL genes were different, the individuals of the three species grouped together. Hence, J. sambac, J. grandiflorum and J. nitidum can be identified using marK and rbcL gene. Further research to identify and compare with other Jasminum species is needed.