Sensitivity evaluation of SYBR green I, SYBR safe and calcein dyes for detection of human papillomavirus 16 by loop-mediated isothermal amplification

• Main Authors: Suharni Mohamad, Nuur Ezzatyhusna Mk, Nurul Izzati Hassan, Siti Suraiya MN, Suharni M
• Format: Article
• Language: English
• Published: Pusat Perubatan Universiti Kebangsaan Malaysia 2017
• Online Access: http://journalarticle.ukm.my/12682/1/7_nuur_et_al_pdf_56028.pdf; http://journalarticle.ukm.my/12682/; http://www.medicineandhealthukm.com/toc/12/2
• Institution: Universiti Kebangsaan Malaysia

Loop-mediated isothermal amplification (LAMP) is a gene amplification technique whereby the amplification products are commonly visualized as turbidity by naked eye in the presence of magnesium pyrophosphate precipitation. An appropriate intercalating dye is important as it could increase the sensit...