PEG-4000 increased the mating efficiency of yeast-two hybrid screening process using PmF-box1 as bait

• Main Authors: Nur Athirah Abd Hamid, Ismanizan Ismail
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2018
• Online Access: http://journalarticle.ukm.my/12916/1/04%20Nur%20Athirah%20Abd%20Hamid.pdf; http://journalarticle.ukm.my/12916/; http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
• Institution: Universiti Kebangsaan Malaysia

Protein degradation can occur through Ubiquitin 26S-Proteosome System (UPS). The degradation can be mediated by the SCF E3 ubiquitin ligase complex consisting of Skp1, Cullin, and F-box protein as the main components. The F-box protein at the C-terminal domain functions to recognize the targeted pro...