Rapid Assembly of yeast expression cassettes for phenylpropanoid biosynthesis in Saccharomyces cerevisiae

• Main Authors: Ahmad Bazli Ramzi, Ku Nurul Aqmar Ku Bahaudin, Syarul Nataqain Baharum, Muhammad Lutfi Che Me, Goh, Hoe-Han, Maizom Hassan, Normah Mohd Noor
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2018
• Online Access: http://journalarticle.ukm.my/12917/1/05%20Ahmad%20Bazli%20Ramzi.pdf; http://journalarticle.ukm.my/12917/; http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
• Institution: Universiti Kebangsaan Malaysia

Microbial production of natural products using metabolic engineering and synthetic biology approaches often involves the assembly of multiple gene fragments including regulatory elements, especially when using eukaryotes as hosts. Traditional cloning strategy using restriction enzyme digestion and l...