Anti-inflammatory effect of tamarind seed coat extract against LPS-induced RAW264.7 macrophages
Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various co...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15465/1/8.pdf http://journalarticle.ukm.my/15465/ http://www.ukm.my/jsm/malay_journals/jilid49bil6_2020/KandunganJilid49Bil6_2020.html |
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| Institution: | Universiti Kebangsaan Malaysia |
| Language: | English |
| Summary: | Inflammatory response is modulated by stimulated immune cells, and has a pivotal role in host defense system against
various stimuli. In this study, we evaluated the anti-inflammatory property of tamarind seed coat extract (TSCE) in
lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Various concentrations of TSCE (10, 25, and 50 µg mL-1)
were applied and then stimulated with LPS (1 μg mL-1) in RAW264.7 macrophages and the level of reactive oxygen
species (ROS) and nitric oxide (NO) were measured. Besides, enzyme-linked immunosorbent assay (ELISA) was used to
measure the level of pro-inflammatory cytokines. Our results showed that TSCE suppressed LPS-induced intracellular
ROS production and suppressed the NO levels in a dose-dependent manner. Significantly, the anti-inflammatory activity
was correlated with a lowered LPS-stimulated TNF-α and IL-1β pro-inflammatory cytokines. These results implied
that TSCE possess potent anti-inflammatory activity, which supported new insights into the TSCE utilization to protect
inflammation-related disorders. |
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