Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress

Nannochloropsis oculata is a unicellular microalgae which is vastly found throughout the environment and have been widely studied due to its high productivity of secondary metabolites and oil content. It is majorly cultured in the aquaculture sector as fish feed and for industries for its polyunsatu...

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Main Authors: Aisamuddin Ardi Zainal Abidin, Zetty Norhana Balia Yusof, Chotika Yokthongwattana
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/16492/1/5.pdf
http://journalarticle.ukm.my/16492/
https://www.ukm.my/jsm/malay_journals/jilid50bil2_2021/KandunganJilid50Bil2_2021.html
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Institution: Universiti Kebangsaan Malaysia
Language: English
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spelling my-ukm.journal.164922021-04-28T04:22:37Z http://journalarticle.ukm.my/16492/ Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress Aisamuddin Ardi Zainal Abidin, Zetty Norhana Balia Yusof, Chotika Yokthongwattana, Nannochloropsis oculata is a unicellular microalgae which is vastly found throughout the environment and have been widely studied due to its high productivity of secondary metabolites and oil content. It is majorly cultured in the aquaculture sector as fish feed and for industries for its polyunsaturated fatty acids. This work aims to study the impact of salinity and oxidative stress on the expression of carotenoid biosynthesis genes and the accumulation of their products in N. oculata via qPCR and HPLC analyses. Three genes responsible for production of high value carotenoids namely lycopene beta-cyclase (CrTL-B/LCYB), beta-carotene oxygenase (CrTO) and beta-carotene hydroxylase (CrTR) under different stresses and time points were identified and quantified, and the amount of their products namely β-carotene, zeaxanthin, canthaxanthin, and astaxanthin was measured. N. oculata was treated with different concentrations of Cu2+ ion (1, 2, and 5 ppm) and NaCl (50, 150, 250 mM) which resembles conditions of oxidative and salinity stress, respectively. RNA and carotenoids extraction, RT-PCR, qPCR and HPLC was carried out in order to identify the correlation of carotenogenesis genes expression with carotenoids production. Under exposure of both treatments, the carotenoids biosynthesis genes were upregulated up to 6-fold compared to control and targeted carotenoids were overexpressed up to 7-fold. Results from this study gave insights which are beneficial in understanding microalgae’s responses towards abiotic stress via the synthesis of carotenoids. Penerbit Universiti Kebangsaan Malaysia 2021-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16492/1/5.pdf Aisamuddin Ardi Zainal Abidin, and Zetty Norhana Balia Yusof, and Chotika Yokthongwattana, (2021) Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress. Sains Malaysiana, 50 (2). pp. 327-337. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil2_2021/KandunganJilid50Bil2_2021.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Nannochloropsis oculata is a unicellular microalgae which is vastly found throughout the environment and have been widely studied due to its high productivity of secondary metabolites and oil content. It is majorly cultured in the aquaculture sector as fish feed and for industries for its polyunsaturated fatty acids. This work aims to study the impact of salinity and oxidative stress on the expression of carotenoid biosynthesis genes and the accumulation of their products in N. oculata via qPCR and HPLC analyses. Three genes responsible for production of high value carotenoids namely lycopene beta-cyclase (CrTL-B/LCYB), beta-carotene oxygenase (CrTO) and beta-carotene hydroxylase (CrTR) under different stresses and time points were identified and quantified, and the amount of their products namely β-carotene, zeaxanthin, canthaxanthin, and astaxanthin was measured. N. oculata was treated with different concentrations of Cu2+ ion (1, 2, and 5 ppm) and NaCl (50, 150, 250 mM) which resembles conditions of oxidative and salinity stress, respectively. RNA and carotenoids extraction, RT-PCR, qPCR and HPLC was carried out in order to identify the correlation of carotenogenesis genes expression with carotenoids production. Under exposure of both treatments, the carotenoids biosynthesis genes were upregulated up to 6-fold compared to control and targeted carotenoids were overexpressed up to 7-fold. Results from this study gave insights which are beneficial in understanding microalgae’s responses towards abiotic stress via the synthesis of carotenoids.
format Article
author Aisamuddin Ardi Zainal Abidin,
Zetty Norhana Balia Yusof,
Chotika Yokthongwattana,
spellingShingle Aisamuddin Ardi Zainal Abidin,
Zetty Norhana Balia Yusof,
Chotika Yokthongwattana,
Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
author_facet Aisamuddin Ardi Zainal Abidin,
Zetty Norhana Balia Yusof,
Chotika Yokthongwattana,
author_sort Aisamuddin Ardi Zainal Abidin,
title Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
title_short Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
title_full Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
title_fullStr Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
title_full_unstemmed Carotenogenesis in Nannochloropsis oculata under oxidative and salinity stress
title_sort carotenogenesis in nannochloropsis oculata under oxidative and salinity stress
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/16492/1/5.pdf
http://journalarticle.ukm.my/16492/
https://www.ukm.my/jsm/malay_journals/jilid50bil2_2021/KandunganJilid50Bil2_2021.html
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